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Oral immunisation of chickens using cholera toxin B subunit and Softigen (R) as adjuvants results in high antibody titre in the egg yolk
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Comparative Medicine. (Hau)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Comparative Medicine. (Hau)
2001 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 15, no 5, 381-384 p.Article in journal (Refereed) Published
Abstract [en]

Oral immunisation by gavage of laying hens with human immunoglobulin G (IgG) combined with a number of potential adjuvants was performed. The resulting immunospecific egg yolk (IgY) antibodies were quantified by ELISA. The following adjuvants were tested: A Poly(lactide-co-glycolide) (PLG) microspheres, Cholera toxin B-subunit (CTB), CTB conjugated with glutaraldehyde, Dimethyl dioctadecyl ammonium bromide (DDA), and Softigen(R) (pegylated C8/C10 mono/di glyceride). Hens in a positive control group were immunised with human IgG in saline emulsified with an equal volume of Freund's Incomplete Adjuvant. High titres of immunospecific IgY antibodies against human IgG were recorded in the eggs from the chickens immunised orally, with the antigen combined with glutaraldehyde conjugated CTB and in the chickens immunised with the antigen combined with Softigen. The present results show that invasive technique related stress could be eliminated/reduced in polyclonal antibody producing animals.

Place, publisher, year, edition, pages
2001. Vol. 15, no 5, 381-384 p.
Keyword [en]
chicken, hen, oral immunisation, cholera toxin, pegylated C8/C10 mono/diglyceride, softigen, egg yolk antibody, immunoglobulin Y, IgY, animal welfare
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-59664OAI: oai:DiVA.org:uu-59664DiVA: diva2:87574
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-01Bibliographically approved

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Persdotter Hedlund, Gabriella

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