uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions
Tsinghua Univ, Sch Life Sci, Struct Biol Ctr, Key Lab Prot Sci,Minist Educ, Beijing 100084, Peoples R China..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Tsinghua Univ, Sch Life Sci, Struct Biol Ctr, Key Lab Prot Sci,Minist Educ, Beijing 100084, Peoples R China..
Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China..
Show others and affiliations
2015 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 22, no 11, 906-913 p.Article in journal (Refereed) Published
Abstract [en]

Adverse cellular conditions often lead to nonproductive translational stalling and arrest of ribosomes on mRNAs. Here, we used fast kinetics and cryo-EM to characterize Escherichia coil HflX, a GTPase with unknown function. Our data reveal that HflX is a heat shock-induced ribosome-splitting factor capable of dissociating vacant as well as mRNA-associated ribosomes with deacylated tRNA in the peptidyl site. Structural data demonstrate that the N-terminal effector domain of HflX binds to the peptidyl transferase center in a strikingly similar manner as that of the class I release factors and induces dramatic conformational changes in central intersubunit bridges, thus promoting subunit dissociation. Accordingly, loss of HflX results in an increase in stalled ribosomes upon heat shock, These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.

Place, publisher, year, edition, pages
2015. Vol. 22, no 11, 906-913 p.
National Category
Cell Biology
URN: urn:nbn:se:uu:diva-268420DOI: 10.1038/nsmb.3103ISI: 000364273800014PubMedID: 26458047OAI: oai:DiVA.org:uu-268420DiVA: diva2:877200
Swedish Research Council, 2010-2619Swedish Research Council, 2011-6088Swedish Research Council, 2014-4423Swedish Research Council, 2008-6593Knut and Alice Wallenberg Foundation, KAW 2011.0081
Available from: 2015-12-06 Created: 2015-12-04 Last updated: 2016-02-08

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Mandava, Chandra SekharSanyal, Suparna
By organisation
Structure and Molecular Biology
In the same journal
Nature Structural & Molecular Biology
Cell Biology

Search outside of DiVA

GoogleGoogle ScholarTotal: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 225 hits
ReferencesLink to record
Permanent link

Direct link