uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris
Univ Helsinki, Dept Forest Sci, FIN-00014 Helsinki, Finland..
Univ Helsinki, Dept Forest Sci, FIN-00014 Helsinki, Finland..
Univ Helsinki, Dept Forest Sci, FIN-00014 Helsinki, Finland..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Show others and affiliations
2015 (English)In: Fungal Genetics and Biology, ISSN 1087-1845, E-ISSN 1096-0937, Vol. 84, 41-51 p.Article in journal (Refereed) Published
Abstract [en]

The cerato-platanin family is a group of small secreted cysteine-rich proteins exclusive for filamentous fungi. They have been shown to be involved in the interactions between fungi and plants. Functional characterization of members from this family has been performed mainly in Ascomycota, except Moniliophthora perniciosa. Our previous phylogenetic analysis revealed that recent gene duplication of cerato-platanins has occurred in Basidiomycota but not in Ascomycota, suggesting higher functional diversification of this protein family in Basidiomycota than in Ascomycota. In this study, we identified three cerato-platanin homologues from the basidiomycete conifer pathogen Heterobasidion annosum sensu stricto. Expression of the homologues under various conditions as well as their roles in the H. annosum s.s.-Pinus sylvestris (Scots pine) pathosystem was investigated. Results showed that HaCPL2 (ceratoplatanin-like protein 2) had the highest sequence similarity to cerato-platanin from Ceratocystis platani and hacpl2 was significantly induced during nutrient starvation and necrotrophic growth. The treatment with recombinant HaCPL2 induced cell death, phytoalexin production and defense gene expression in Nicotiana tabacum. Eliciting and cell death-inducing ability accompanied by retardation of apical root growth was also demonstrated in Scots pine seedlings. Our results suggest that HaCPL2 might contribute to the virulence of H. annosum s.s. by promoting plant cell death.

Place, publisher, year, edition, pages
2015. Vol. 84, 41-51 p.
Keyword [en]
Fungal elicitor, Fungal effector, Phytotoxicity, Virulence, Plant defense
National Category
Botany Cell Biology
URN: urn:nbn:se:uu:diva-268413DOI: 10.1016/j.fgb.2015.09.007ISI: 000364263100005OAI: oai:DiVA.org:uu-268413DiVA: diva2:877263
Available from: 2015-12-06 Created: 2015-12-04 Last updated: 2015-12-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Structure and Molecular Biology
In the same journal
Fungal Genetics and Biology
BotanyCell Biology

Search outside of DiVA

GoogleGoogle ScholarTotal: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 262 hits
ReferencesLink to record
Permanent link

Direct link