uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Intestinal DMT1 is critical for iron absorption in the mouse but is not required for the absorption of copper or manganese
Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, Cincinnati, OH 45220 USA.;Univ Cincinnati, Coll Med, Syst Biol & Physiol Program, Cincinnati, OH USA..
Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, Cincinnati, OH 45220 USA..
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC USA..
Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, Cincinnati, OH 45220 USA.;Univ Cincinnati, Coll Med, Syst Biol & Physiol Program, Cincinnati, OH USA..
Show others and affiliations
2015 (English)In: American Journal of Physiology - Gastrointestinal and Liver Physiology, ISSN 0193-1857, E-ISSN 1522-1547, Vol. 309, no 8, G635-G647 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Divalent metal-ion transporter-1 (DMT1) is a widely expressed iron-preferring membrane-transport protein that serves a critical role in erythroid iron utilization. We have investigated its role in intestinal metal absorption by studying a mouse model lacking intestinal DMT1 (i.e., DMT1(int/int)). DMT1(int/int) mice exhibited a profound hypochromic-microcytic anemia, splenomegaly, and cardiomegaly. That the anemia was due to iron deficiency was demonstrated by the following observations in DMT1(int/int) mice: 1) blood iron and tissue nonheme-iron stores were depleted; 2) mRNA expression of liver hepcidin (Hamp1) was depressed; and 3) intraperitoneal iron injection corrected the anemia, and reversed the changes in blood iron, nonheme-iron stores, and hepcidin expression levels. We observed decreased total iron content in multiple tissues from DMT1(int/int) mice compared with DMT1(+/+) mice but no meaningful change in copper, manganese, or zinc. DMT1(int/int) mice absorbed Cu-64 and Mn-54 from an intragastric dose to the same extent as did DMT1(+/+) mice but the absorption of Fe-59 was virtually abolished in DMT1(int/int) mice. This study reveals a critical function for DMT1 in intestinal nonheme-iron absorption for normal growth and development. Further, this work demonstrates that intestinal DMT1 is not required for the intestinal transport of copper, manganese, or zinc.

Place, publisher, year, edition, pages
2015. Vol. 309, no 8, G635-G647 p.
Keyword [en]
copper absorption, iron deficiency, iron-deficiency anemia, iron-refractive iron-deficiency anemia, manganese absorption, SLC11A2, zinc metabolism
National Category
Gastroenterology and Hepatology Physiology
Identifiers
URN: urn:nbn:se:uu:diva-268700DOI: 10.1152/ajpgi.00160.2015ISI: 000364068300003PubMedID: 26294671OAI: oai:DiVA.org:uu-268700DiVA: diva2:880542
Available from: 2015-12-09 Created: 2015-12-09 Last updated: 2018-01-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Öhrvik, Helena

Search in DiVA

By author/editor
Öhrvik, Helena
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
American Journal of Physiology - Gastrointestinal and Liver Physiology
Gastroenterology and HepatologyPhysiology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 572 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf