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The Human Endometrium-Specific Proteome Defined by Transcriptomics and Antibody-Based Profiling
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Obstetrics and Gynaecology. Uppsala Univ, Dept Womens & Childrens Hlth, S-75185 Uppsala, Sweden..
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
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2015 (English)In: Omics, ISSN 1536-2310, E-ISSN 1557-8100, Vol. 19, no 11, 659-668 p.Article in journal (Refereed) Published
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Abstract [en]

The human uterus includes the complex endometrial mucosa, the endometrium that undergoes dynamic, hormone-dependent alterations throughout the life of fertile females. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to analyze gene expression patterns in the normal endometrium. Human endometrial tissues from five women were used for deep sequencing (RNA-Seq). The mRNA and protein expression data from the endometrium were compared to 31 (RNA) and 44 (protein) other normal tissue types, to identify genes with elevated expression in the endometrium and to localize the expression of corresponding proteins at a cellular resolution. Based on the expression levels of transcripts, we could classify all putative human protein coding genes into categories defined by expression patterns and found altogether 101 genes that showed an elevated pattern of expression in the endometrium, with only four genes showing more than five-fold higher expression levels in the endometrium compared to other tissues. In conclusion, our analysis based on transcriptomics and antibody-based protein profiling reports here comprehensive lists of genes with elevated expression levels in the endometrium, providing important starting points for a better molecular understanding of human reproductive biology and disease.

Place, publisher, year, edition, pages
2015. Vol. 19, no 11, 659-668 p.
National Category
Medical Genetics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-269256DOI: 10.1089/omi.2015.0115ISI: 000364547500001PubMedID: 26488136OAI: oai:DiVA.org:uu-269256DiVA: diva2:882737
Funder
Knut and Alice Wallenberg Foundation
Available from: 2015-12-15 Created: 2015-12-15 Last updated: 2017-12-01Bibliographically approved

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Zieba, AgataOlovsson, MattsTolf, AnnaPontén, Fredrik

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Molecular toolsObstetrics and GynaecologyDepartment of Immunology, Genetics and Pathology
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Medical GeneticsMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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