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Lineage- CD34hi CD117int/hi FcϵRI+ cells in human blood constitute a rare population of mast cell progenitors
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2016 (English)In: Blood, ISSN 0006-4971, E-ISSN 1528-0020, Vol. 127, no 4, 383-391 p.Article in journal (Refereed) Published
Abstract [en]

Mast cells are rare tissue-resident immune cells that are involved in allergic reactions, and their numbers are increased in the lungs of asthmatics. Murine lung mast cells arise from committed bone marrow-derived progenitors that enter the blood circulation, migrate through the pulmonary endothelium, and mature in the tissue. In humans, mast cells can be cultured from multipotent CD34(+) progenitor cells. However, a population of distinct precursor cells that give rise to mast cells has remained undiscovered. To our knowledge, this is the first report of human lineage(-) CD34(hi) CD117(int/hi) FcϵRI(+) progenitor cells, which represented only 0.0053% of the isolated blood cells in healthy individuals. These cells expressed integrin β7 and developed a mast cell-like phenotype, although with a slow cell division capacity in vitro. Isolated lineage(-) CD34(hi) CD117(int/hi) FcϵRI(+) blood cells had an immature mast cell-like appearance and expressed high levels of many mast cell-related genes as compared with human blood basophils in whole-transcriptome microarray analyses. Furthermore, serglycin, tryptase, and carboxypeptidase A mRNA transcripts were detected by quantitative RT-PCR. Altogether, we propose that the lineage(-) CD34(hi) CD117(int/hi) FcϵRI(+) blood cells are closely related to human tissue mast cells and likely constitute an immediate precursor population, which can give rise to predominantly mast cells. Furthermore, asthmatics with reduced lung function had a higher frequency of lineage(-) CD34(hi) CD117(int/hi) FcϵRI(+) blood mast cell progenitors than asthmatics with normal lung function.

Place, publisher, year, edition, pages
2016. Vol. 127, no 4, 383-391 p.
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Medical and Health Sciences Hematology
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URN: urn:nbn:se:uu:diva-270369DOI: 10.1182/blood-2015-06-650648ISI: 000369288300007PubMedID: 26626992OAI: oai:DiVA.org:uu-270369DiVA: diva2:889628
Available from: 2015-12-27 Created: 2015-12-27 Last updated: 2017-12-01

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Malinovschi, AndreiÖhrvik, HelenaSandelin, MartinJanson, ChristerAlving, KjellHallgren, Jenny

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Malinovschi, AndreiÖhrvik, HelenaSandelin, MartinJanson, ChristerAlving, KjellHallgren, Jenny
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Department of Medical Biochemistry and MicrobiologyClinical PhysiologyDepartment of Medical SciencesDepartment of Women's and Children's Health
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Blood
Medical and Health SciencesHematology

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