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Removal of xenotropic murine leukemia virus by nanocellulose based filter paper
Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany..
Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany..
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
2015 (English)In: Biologicals (Print), ISSN 1045-1056, E-ISSN 1095-8320, Vol. 43, no 6, 452-456 p.Article in journal (Refereed) Published
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Abstract [en]

The removal of xenotrpic murine leukemia virus (xMuLV) by size-exclusion filter paper composed of 100% naturally derived cellulose was validated. The filter paper was produced using cellulose nanofibers derived from Cladophora sp. algae. The filter paper was characterized using atomic force microscopy, scanning electron microscopy, helium pycnometry, and model tracer (100 nm latex beads and 50 nm gold nanoparticles) retention tests. Following the filtration of xMuLV spiked solutions, LRV >= 5.25 log(10) TCID50 was observed, as limited by the virus titre in the feed solution and sensitivity of the tissue infectivity test. The results of the validation study suggest that the nanocellulose filter paper is useful for removal of endogenous rodent retroviruses and retrovirus-like particles during the production of recombinant proteins.

Place, publisher, year, edition, pages
2015. Vol. 43, no 6, 452-456 p.
Keyword [en]
Viral contamination, Virus retentive filtration, Cladophora cellulose, Paper making, Recombinant proteins and monoclonal antibodies
National Category
Nano Technology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-270621DOI: 10.1016/j.biologicals.2015.08.001ISI: 000365370400003PubMedID: 26328471OAI: oai:DiVA.org:uu-270621DiVA: diva2:890232
Funder
Knut and Alice Wallenberg Foundation, KAW 2013.0190Göran Gustafsson Foundation for promotion of scientific research at Uppala University and Royal Institute of Technology, 1360B
Available from: 2016-01-01 Created: 2016-01-01 Last updated: 2017-12-01Bibliographically approved

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Mihranyan, Albert

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