uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Knockdown of Tmem234 in zebrafish results in proteinuria
Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden.;Karolinska Univ, Huddinge Hosp, Karolinska Inst, KI AZ Integrated CardioMetab Ctr,Dept Med, Stockholm, Sweden..
Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden..
Karolinska Univ, Huddinge Hosp, Karolinska Inst, KI AZ Integrated CardioMetab Ctr,Dept Med, Stockholm, Sweden..
Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden..
Show others and affiliations
2015 (English)In: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, ISSN 1931-857X, Vol. 309, no 11, F955-F966 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Podocytes are highly specialized epithelial cells located at the outer aspects of the glomerular capillary tuft and critical components of the kidney filtration barrier. To maintain their unique features, podocytes express a number of proteins that are only sparsely found elsewhere in the body. In this study, we have identified four (Tmem234, Znf185, Lrrc49, and Slfn5) new highly podocyte-enriched proteins. The proteins are strongly expressed by podocytes, while other parts of the kidney show only weak or no expression. Tmem234, Slfn5, and Lrrc49 are located in foot processes, whereas Znf185 is found in both foot and major processes. Expressional studies in developing kidneys show that these proteins are first expressed at the capillary stage glomerulus, the same stage when the formation of major and foot processes begins. We identified zebrafish orthologs for Tmem234 and Znf185 genes and knocked down their expression using morpholino technology. Studies in zebrafish larvae indicate that Tmem234 is essential for the organization and functional integrity of the pronephric glomerulus filtration barrier, as inactivation of Tmem234 expression results in foot process effacement and proteinuria. In summary, we have identified four novel highly podocyte - enriched proteins and show that one of them, Tmem234, is essential for the normal filtration barrier in the zebrafish pronephric glomerulus. Identification of new molecular components of the kidney filtration barrier opens up possibilities to study their role in glomerulus biology and diseases.

Place, publisher, year, edition, pages
2015. Vol. 309, no 11, F955-F966 p.
Keyword [en]
podocyte, pronephros, podocin, Tmem234
National Category
Urology and Nephrology Physiology
Identifiers
URN: urn:nbn:se:uu:diva-270934DOI: 10.1152/ajprenal.00525.2014ISI: 000365897700008PubMedID: 26377798OAI: oai:DiVA.org:uu-270934DiVA: diva2:890935
Funder
Swedish Research CouncilKnut and Alice Wallenberg FoundationSwedish Diabetes AssociationSwedish Child Diabetes FoundationStockholm County CouncilThe Karolinska Institutet's Research Foundation
Available from: 2016-01-05 Created: 2016-01-05 Last updated: 2016-01-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Betsholtz, Christer

Search in DiVA

By author/editor
Betsholtz, Christer
By organisation
Vascular Biology
Urology and NephrologyPhysiology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 256 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf