Biobanking multifocal breast carcinomas: sample adequacy with regard to histology and DNA content
2016 (English)In: Histopathology, ISSN 0309-0167, E-ISSN 1365-2559, Vol. 68, no 3, 411-421 p.Article in journal (Refereed) Published
AIMS: To determine the volume of tumoral and normal breast tissue containing sufficient DNA (>2 μg/sample) for genetic platforms and biobanking, with a focus on multifocality, tumoral heterogeneity, and factors that critically influence sample acceptability.
METHODS AND RESULTS: We examined 57 breast surgical specimens with multifocal (46/57) and unifocal (11/57) cancers. Punch biopsies were obtained from tissue slices under multimodal radiological guidance, and the colour-coded sampling sites were identified in large-format histology slides. The study comprised 415 DNA isolations from tumour (n = 105) and normal (n = 283) tissue, including skin (n = 27) samples. A single 2-mm core from invasive tumour contained sufficient DNA in 91.4% (96/105) of cases, depending on tumour type (3.8-108.2 μg/sample), number and size of additional foci in multifocal cases (P = 0.001), tumour consistency, and degree of necrosis. Three biopsies obtained with a 4-mm device were required from normal breast tissue, at least 10 mm from the tumour. Cold ischaemia for up to 82 min did not influence the yield of DNA.
CONCLUSIONS: Radiological disease mapping is useful for guiding optimal specimen slicing and for targeting breast lesions. A single 2-mm core from tumour and multiple 4-mm cores from normal breast tissue yield adequate DNA in the majority of samples.
Place, publisher, year, edition, pages
2016. Vol. 68, no 3, 411-421 p.
biobanking; breast cancer; DNA quality and quantity; heterogeneity; multifocality
Medical and Health Sciences Cancer and Oncology Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-271418DOI: 10.1111/his.12758ISI: 000367819400011PubMedID: 26083274OAI: oai:DiVA.org:uu-271418DiVA: diva2:891869