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Biobanking multifocal breast carcinomas: sample adequacy with regard to histology and DNA content
Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik.ORCID iD: 0000-0002-1701-755X
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2016 (English)In: Histopathology, ISSN 0309-0167, E-ISSN 1365-2559, Vol. 68, no 3, 411-421 p.Article in journal (Refereed) Published
Abstract [en]

AIMS: To determine the volume of tumoral and normal breast tissue containing sufficient DNA (>2 μg/sample) for genetic platforms and biobanking, with a focus on multifocality, tumoral heterogeneity, and factors that critically influence sample acceptability.

METHODS AND RESULTS: We examined 57 breast surgical specimens with multifocal (46/57) and unifocal (11/57) cancers. Punch biopsies were obtained from tissue slices under multimodal radiological guidance, and the colour-coded sampling sites were identified in large-format histology slides. The study comprised 415 DNA isolations from tumour (n = 105) and normal (n = 283) tissue, including skin (n = 27) samples. A single 2-mm core from invasive tumour contained sufficient DNA in 91.4% (96/105) of cases, depending on tumour type (3.8-108.2 μg/sample), number and size of additional foci in multifocal cases (P = 0.001), tumour consistency, and degree of necrosis. Three biopsies obtained with a 4-mm device were required from normal breast tissue, at least 10 mm from the tumour. Cold ischaemia for up to 82 min did not influence the yield of DNA.

CONCLUSIONS: Radiological disease mapping is useful for guiding optimal specimen slicing and for targeting breast lesions. A single 2-mm core from tumour and multiple 4-mm cores from normal breast tissue yield adequate DNA in the majority of samples.

Place, publisher, year, edition, pages
2016. Vol. 68, no 3, 411-421 p.
Keyword [en]
biobanking; breast cancer; DNA quality and quantity; heterogeneity; multifocality
National Category
Medical and Health Sciences Cancer and Oncology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-271418DOI: 10.1111/his.12758ISI: 000367819400011PubMedID: 26083274OAI: oai:DiVA.org:uu-271418DiVA: diva2:891869
Available from: 2016-01-07 Created: 2016-01-07 Last updated: 2017-12-01Bibliographically approved

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Pekar, GyulaDavies, HannaForsberg, LarsHellberg, DanDumanski, Jan

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Department of Immunology, Genetics and PathologyMedicinsk genetik och genomikCenter for Clinical Research DalarnaDepartment of Women's and Children's Health
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Histopathology
Medical and Health SciencesCancer and OncologyCell and Molecular Biology

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