Simvastatin and zinc synergistically enhance osteoblasts activityand decrease the acute response of inflammatory cells
2016 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 27, no 2, 23Article in journal (Refereed) Published
Several ceramic biomaterials have been suggested as promising alternatives to autologous bone to replace or restore bone after trauma or disease. The osteoinductive potential of most scaffolds is often rather low by themselves and for this reason growth factors or drugs have been supplemented to these synthetic materials. Although some growth factors show good osteoinductive potential their drawback is their high cost and potential severe side effects. In this work the combination of the well-known drug simvastatin (SVA) and the inorganic element Zinc (Zn) is suggested as a potential additive to bone grafts in order to increase their bone regeneration/ formation. MC3T3-E1 cells were cultured with Zn (10 and 25 mu M) and SVA (0.25 and 0.4 mu M) for 10 days to evaluate proliferation and differentiation, and for 22 days to evaluate secretion of calcium deposits. The combination of Zn (10 mu M) and SVA (0.25 mu M) significantly enhanced cell differentiation and mineralization in a synergetic manner. In addition, the release of reactive oxygen species (ROS) from primary human monocytes in contact with the same concentrations of Zn and SVA was evaluated by chemiluminescence. The combination of the additives decreased the release of ROS, although Zn and SVA separately caused opposite effects. This work shows that a new combination of additives can be used to increase the osteoinductive capacity of porous bioceramics.
Place, publisher, year, edition, pages
2016. Vol. 27, no 2, 23
Engineering and Technology Bio Materials
IdentifiersURN: urn:nbn:se:uu:diva-271530DOI: 10.1007/s10856-015-5639-4ISI: 000368726300002PubMedID: 26704540OAI: oai:DiVA.org:uu-271530DiVA: diva2:892228
FunderEU, FP7, Seventh Framework Programme, GROWTH 291795VINNOVA, 2013-01260Lars Hierta Memorial Foundation