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Chondroitinase AC: a host-associated genetic feature of Helicobacter bizzozeronii
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. (Glycobiology)ORCID iD: 0000-0003-4295-2395
INRA, ChemSyBio, UMR Micalis 1319, F-78350 Jouy En Josas, France;AgroParisTech, ChemSyBio, UMR Micalis, F-78350 Jouy En Josas, France.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Univ Helsinki, Dept Food Hyg & Environm Hlth, Fac Vet Med, POB 66,Agnes Sjobergin Katu 2, FI-00014 Helsinki, Finland.
2016 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 186, 21-27 p.Article in journal (Refereed) Published
Abstract [en]

Investigating mechanisms involved in host adaptation is crucial to understand pathogen evolution. Helicobacter species appear to have a host species-specific tropism, coevolving with their natural hosts, and to develop several strategies allowing the colonization of the stomach throughout lifetime of their hosts. However, little is known about genetic features associated with the adaptation to a specific animal host. In this study we discovered a polysaccharide lyase that is expressed by the canine-associated species H. bizzozeronii and acts as chondroitinase AC-type lyase of broad specificity. Except for its low pH optimum between pH 4.0 and pH 5.5, the properties of the H. bizzozeronii chondroitin lyase AC resemble the ones from Arthrobacter aurescens. However, homologues of this gene have been detected only in Helicobacter species colonizing the canine and feline gastric mucosa. Since a unique feature of the canine stomach is the secretion of chondroitin-4-sulphate in the gastric juice of the fundus mucosa by chief cells, the expression of chondroitinase AC by H. bizzozeronii is likely the consequence of adaptation of this bacterium to its host and a potential link to gastric disorders in dogs.

Place, publisher, year, edition, pages
2016. Vol. 186, 21-27 p.
Keyword [en]
Canine gut microbiota; Helicobactor bizzozeronii; Glycosaminoglycan lyase; Carbohydrate lyases; Chondroitin sulphate
National Category
Microbiology in the medical area Cell and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry; Microbiology; Molecular Cellbiology; Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-272079DOI: 10.1016/j.vetmic.2016.02.013ISI: 000374709900004PubMedID: 27016753OAI: oai:DiVA.org:uu-272079DiVA: diva2:892948
Available from: 2016-01-11 Created: 2016-01-11 Last updated: 2017-11-30Bibliographically approved
In thesis
1. Brittlestars Galactosaminoglycans and Tools to Study their Structure
Open this publication in new window or tab >>Brittlestars Galactosaminoglycans and Tools to Study their Structure
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In all living organisms, biological activities such as proper functioning and co-ordination of different organs will depend on different cells and molecular interactions. In some organisms the loss of functional organs or damage of organs can be lethal, whereas in others a special process called regeneration can retrieve lost organs. The molecular details of regeneration are still not completely understood in many organisms. Echinoderms are close to vertebrates in the evolutionary tree and are well known for their amazing regeneration capacity. So we chose to investigate the molecular processes of regeneration mechanism with an interest towards our favorite groups of molecules, glycosaminoglycans (GAGs). GAGs are linear polysaccharides, expressed on all cell surfaces and extracellular space and are also known to be involved in many cellular activities. We aimed to characterize the GAGs present in Echinodermata species Amphiura filiformis and investigated their role during arm regeneration.

In Paper I we characterized the structure and function of GAGs from A. filiformis and identified that A. filiformis contains CS/DS type of GAGs, but no HS. The sulfation degree of these CS/DS is close to the one of heparin, i.e. they are highly sulfated. These chains are able to bind FGF-2 growth factor and induce FGF-2 mediated cell signaling. In Paper II we further characterized these GAGs for their localization and for their role in arm regeneration in A. filiformis. Immuno- and histochemical stainings on arm sections revealed that CS/DS GAGs are localized around the podia, surrounding the water vascular system, and around the muscle tissues. Inhibition of sulfated GAG biosynthesis by chlorate treatment affected the regeneration efficiency of the arms, which may be an indication of the importance of CS/DS structures in A. filiformis arm regeneration. We also characterized some bacterial sulfatases in Paper III and a lyase in Paper IV from human and canine gut symbiotic bacteria. Here we sought to find the substrate specificity and optimal conditions for these enzymes’ activities. Our findings suggest that these polysaccharide lyase and sulfatases can be used as potential tools to characterize different GAG structures and their application could further add knowledge on diseases mechanisms related to host pathogen interactions.

 

 

 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2016. 63 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 1172
Keyword
Chondroitin sulfate, Brittlestars, Gut bacteria, H. bizzozeronii, B.thetaiotaomicron
National Category
Microbiology in the medical area Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cell and Molecular Biology
Research subject
Biochemistry; Microbiology; Molecular Biology
Identifiers
urn:nbn:se:uu:diva-271559 (URN)978-91-554-9449-0 (ISBN)
Public defence
2016-02-26, A1: 107, BMC, Husargatan 3, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2016-02-02 Created: 2016-01-10 Last updated: 2016-02-12

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Namburi, Ramesh BabuSpillman, Dorothe

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Microbiology in the medical areaCell and Molecular BiologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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