Chondroitinase AC: a host-associated genetic feature of Helicobacter bizzozeronii
2016 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 186, 21-27 p.Article in journal (Other academic) Published
Investigating mechanisms involved in host adaptation is crucial to understand pathogen evolution. Helicobacter species appear to have a host species-specific tropism, coevolving with their natural hosts, and to develop several strategies allowing the colonization of the stomach throughout lifetime of their hosts. However, little is known about genetic features associated with the adaptation to a specific animal host. In this study we discovered a polysaccharide lyase that is expressed by the canine-associated species H. bizzozeronii and acts as chondroitinase AC-type lyase of broad specificity. Except for its low pH optimum between pH 4.0 and pH 5.5, the properties of the H. bizzozeronii chondroitin lyase AC resemble the ones from Arthrobacter aurescens. However, homologues of this gene have been detected only in Helicobacter species colonizing the canine and feline gastric mucosa. Since a unique feature of the canine stomach is the secretion of chondroitin-4-sulphate in the gastric juice of the fundus mucosa by chief cells, the expression of chondroitinase AC by H. bizzozeronii is likely the consequence of adaptation of this bacterium to its host and a potential link to gastric disorders in dogs.
Place, publisher, year, edition, pages
2016. Vol. 186, 21-27 p.
Canine gut microbiota; Helicobactor bizzozeronii; Glycosaminoglycan lyase; Carbohydrate lyases; Chondroitin sulphate
Microbiology in the medical area Cell and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject Biochemistry; Microbiology; Molecular Cellbiology; Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-272079DOI: 10.1016/j.vetmic.2016.02.013ISI: 000374709900004PubMedID: 27016753OAI: oai:DiVA.org:uu-272079DiVA: diva2:892948