PP015. Plasma levels and placental expression of BNP/NT-proBNP in early and late onset preeclampsia
2013 (English)In: Pregnancy Hypertension, ISSN 2210-7789, E-ISSN 2210-7797, Vol. 3, no 2, 73- p.Article in journal, Meeting abstract (Refereed) Published
Women with preeclampsia (PE) have elevated plasma levels of NT-proBNP. We hypothesized that the placenta may be a source to these elevated levels.
Our objectives were to study the plasma levels of NT-proBNP and the protein and mRNA expression of placental BNP in women with early and late onset PE and controls.
Plasma levels of NT-proBNP were measured in women with early (n=18) and late (n=20) onset PE, in two groups of healthy pregnant women in gestational week 24-32 (n=22) and 36-42 (n=14), and in non-pregnant women (n=20). Placental BNP protein and mRNA was studied with immunohistochemistry and qPCR. Placental release of NT-proBNP was studied with tissue culturing.
Women with early (365 (14-9815) pg/ml) and late (176 (33-2547) pg/ml) onset PE had higher levels of NT-proBNP in plasma than their respective controls (p<0.001). A tendency towards higher plasma levels in early compared to late onset PE was observed (p=0.057). 20 out of 25 placental tissue samples had proBNP mRNA, no differences between the study groups were found. BNP protein was found in maternal spiral arteries and syncytiotrophoblasts. NT-proBNP peptide (6-7pg/ml) was present in medium used for placenta cultures.
Our results suggest that there may be a placental source of NT-proBNP. If this source is responsible for the elevated plasma levels of NT-proBNP in preeclamptic women and what role, if any, BNP/NT-proBNP play in PE pathophysiology remains to be elucidated.
Place, publisher, year, edition, pages
2013. Vol. 3, no 2, 73- p.
Obstetrics, Gynecology and Reproductive Medicine
IdentifiersURN: urn:nbn:se:uu:diva-272559DOI: 10.1016/j.preghy.2013.04.043PubMedID: 26105874OAI: oai:DiVA.org:uu-272559DiVA: diva2:894389
ISSHP, 12-14 June 2013, Tromsø, Norway2016-01-142016-01-142016-02-10Bibliographically approved