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Biotin labelling of chicken antibodies and their subsequent use in ELISA and immunohistochemistry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Human Anatomy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.ORCID iD: 0000-0003-3161-0402
1993 (English)In: Comparative Immunology, Microbiology & Infectious Diseases, ISSN 0147-9571, E-ISSN 1878-1667, Vol. 16, no 2, 145-152 p.Article in journal (Refereed) Published
Abstract [en]

Avian antibodies have many advantages to mammalian antibodies due to the phylogenetic differences between birds and mammals, resulting in an increased sensitivity and a decreased background in many immunological assays. Since the avidin-biotin system is an efficient detection system for antibodies with a high sensitivity, we wanted to investigate the activity and unspecific binding of optimally biotin labelled chicken antibodies in ELISA and immunohistochemistry. We report on the conditions for biotinylation of chicken antibodies and that optimally biotinylated antibodies show a high activity and a low background in both ELISA and immunohistochemistry.

Place, publisher, year, edition, pages
1993. Vol. 16, no 2, 145-152 p.
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Clinical Medicine
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URN: urn:nbn:se:uu:diva-272621PubMedID: 8319436OAI: oai:DiVA.org:uu-272621DiVA: diva2:894412
Available from: 2016-01-14 Created: 2016-01-14 Last updated: 2017-11-30Bibliographically approved

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Olovsson, MattsLarsson, Anders

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