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Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2015 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, Vol. 105, e53374Article in journal (Refereed) Published
Abstract [en]

Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.

Place, publisher, year, edition, pages
2015. Vol. 105, e53374
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-274679DOI: 10.3791/53374ISI: 000368573900061OAI: oai:DiVA.org:uu-274679DiVA: diva2:897242
Funder
Swedish Cancer Society, 11 0253, 13 0469Swedish Research Council, K2012-99X-21959-01-3 2006-5038-36531-16
Available from: 2016-01-25 Created: 2016-01-25 Last updated: 2017-11-30Bibliographically approved

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Assadian, FarzanehSandström, KarlLaurell, GöranSvensson, CatharinaAkusjärvi, GöranPunga, Tanel

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Department of Medical Biochemistry and MicrobiologyOtolaryngology and Head and Neck Surgery
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