uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling
Show others and affiliations
2015 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 211, no 6, p. 1177-1192Article in journal (Refereed) Published
Abstract [en]

Vascular endothelial (VE)-cadherin transfers intracellular signals contributing to vascular hemostasis. Signaling through VE-cadherin requires association and activity of different intracellular partners. Yes-associated protein (YAP)/TAZ transcriptional cofactors are important regulators of cell growth and organ size. We show that EPS8, a signaling adapter regulating actin dynamics, is a novel partner of VE-cadherin and is able to modulate YAP activity. By biochemical and imaging approaches, we demonstrate that EPS8 associates with the VE-cadherin complex of remodeling junctions promoting YAP translocation to the nucleus and transcriptional activation. Conversely, in stabilized junctions, 14-3-3-YAP associates with the VE-cadherin complex, whereas Eps8 is excluded. Junctional association of YAP inhibits nuclear translocation and inactivates its transcriptional activity both in vitro and in vivo in Eps8-null mice. The absence of Eps8 also increases vascular permeability in vivo, but did not induce other major vascular defects. Collectively, we identified novel components of the adherens junction complex, and we introduce a novel molecular mechanism through which the VE-cadherin complex controls YAP transcriptional activity.

Place, publisher, year, edition, pages
2015. Vol. 211, no 6, p. 1177-1192
National Category
Basic Medicine Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-274801DOI: 10.1083/jcb.201501089ISI: 000367410600009PubMedID: 26668327OAI: oai:DiVA.org:uu-274801DiVA, id: diva2:897654
Funder
EU, European Research Council, EU-ERC 268870EU, European Research Council, EU-ERC 268836EU, European Research Council, ITN VESSEL 317250German Research Foundation (DFG), SFB829
Available from: 2016-01-26 Created: 2016-01-26 Last updated: 2018-01-10

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Dejana, Elisabetta

Search in DiVA

By author/editor
Dejana, Elisabetta
By organisation
Vascular Biology
In the same journal
Journal of Cell Biology
Basic MedicineCell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 482 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf