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Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation?
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Umea Univ, Dept Chem, S-90187 Umea, Sweden..
2015 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 16, no 8, 16728-16739 p.Article in journal (Refereed) Published
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Abstract [en]

The human copper (Cu) chaperone Atox1 delivers Cu to P-1B type ATPases in the Golgi network, for incorporation into essential Cu-dependent enzymes. Atox1 homologs are found in most organisms; it is a 68-residue ferredoxin-fold protein that binds Cu in a conserved surface-exposed Cys-X-X-Cys (CXXC) motif. In addition to its well-documented cytoplasmic chaperone function, in 2008 Atox1 was suggested to have functionality in the nucleus. To identify new interactions partners of Atox1, we performed a yeast two-hybrid screen with a large human placenta library of cDNA fragments using Atox1 as bait. Among 98 million fragments investigated, 25 proteins were found to be confident interaction partners. Nine of these were uncharacterized proteins, and the remaining 16 proteins were analyzed by bioinformatics with respect to cell localization, tissue distribution, function, sequence motifs, three-dimensional structures and interaction networks. Several of the hits were eukaryotic-specific proteins interacting with DNA or RNA implying that Atox1 may act as a modulator of gene regulation. Notably, because many of the identified proteins contain CXXC motifs, similarly to the Cu transport reactions, interactions between these and Atox1 may be mediated by Cu.

Place, publisher, year, edition, pages
2015. Vol. 16, no 8, 16728-16739 p.
Keyword [en]
copper chaperone, Atox1, transcription factor, two-hybrid screen, bioinformatics
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-274713DOI: 10.3390/ijms160816728ISI: 000366826100002PubMedID: 26213915OAI: oai:DiVA.org:uu-274713DiVA: diva2:899629
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Available from: 2016-02-02 Created: 2016-01-25 Last updated: 2017-11-30Bibliographically approved

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Ohrvik, Helena

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