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In Situ Proximity Ligation Assay (PLA)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. (Functional Pharmacology)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. (Functional Pharmacology)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
2015 (English)In: ELISA: Methods and Protocols / [ed] Hnasko, R, Springer-Verlag New York, 2015, Vol. 1318, 149-159 p.Chapter in book (Refereed)
Abstract [en]

In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. Vol. 1318, 149-159 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1318
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-275303DOI: 10.1007/978-1-4939-2742-5_15ISI: 000375613000016PubMedID: 26160573ISBN: 978-1-4939-2742-5 (print)ISBN: 978-1-4939-2741-8 (print)OAI: oai:DiVA.org:uu-275303DiVA: diva2:899817
Available from: 2016-02-02 Created: 2016-02-02 Last updated: 2016-08-11Bibliographically approved

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Bagchi, SonchitaFredriksson, RobertWallén-Mackenzie, Åsa

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