Microwave-assisted high-speed PCR
2003 (English)In: European Journal of Pharmaceutical Sciences, ISSN 0928-0987, E-ISSN 1879-0720, Vol. 18, no 2, 129-132 p.Article in journal (Refereed) PublishedText
PCR amplification has emerged as a very important tool in biological research. The utility of the PCR is, however, hampered by the fact that it is a slow technique. Faster heating cycles are therefore needed, both to enhance the activity of the enzyme, and to enable shortening of the reaction times. In this paper, polymerase chain reactions with focused microwave irradiation as the source of heat were demonstrated for the first time. Thus, it was established that continuous microwave heating does not terminate the enzymatic function of the polymerase. The results indicate the possibility to shorten the total reaction time. In addition, the technique may give the possibility to perform PCR reactions in millilitre scale. (C) 2002 Elsevier Science B.V. All rights reserved.
Place, publisher, year, edition, pages
Univ Uppsala, Uppsala Biomed Ctr, Dept Organ Pharmaceut Chem, SE-75123 Uppsala, Sweden. CanAg Diagnost AB, Majnabbe Terminal, SE-41455 Gothenburg, Sweden., 2003. Vol. 18, no 2, 129-132 p.
polymerase chain reaction, microwaves, nucleic acids
IdentifiersURN: urn:nbn:se:uu:diva-275414DOI: 10.1016/S0928-0987(02)00252-XISI: 000181427500003PubMedID: 12594005OAI: oai:DiVA.org:uu-275414DiVA: diva2:900284