A suppressive effect of Sp1 recruitment to the first leader 5' splice site region on L4-22K-mediated activation of the adenovirus major late promoter
2015 (English)In: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 210, 133-140 p.Article in journal (Refereed) Published
Transcription from the adenovirus major late promoter (MLP) requires binding of late phase-specific factors to the so-called DE element located approximately 100 base pairs downstream of the MLP transcriptional start site. The adenovirus L4-22K protein binds to the DE element and stimulates transcription from the MLP via a DE sequence-dependent mechanism. Here we use a transient expression approach to show that L4-22K binds to an additional site downstream of the MLP start site, the so-called R1 region, which includes the major late first leader 5' splice site. Binding of L4-22K to R1 has a suppressive effect on MLP transcription. L4-22K binds to the distal part of R1 and stimulates the recruitment of Sp1 and other cellular factors to a site overlapping the first leader 5' splice site. Binding of Sp1 to the 5' splice site region had an inhibitory effect on L4-22K-activated MLP transcription.
Place, publisher, year, edition, pages
2015. Vol. 210, 133-140 p.
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-275814DOI: 10.1016/j.virusres.2015.07.026ISI: 000365455500020PubMedID: 26247419OAI: oai:DiVA.org:uu-275814DiVA: diva2:901325
FunderSwedish Cancer Society, 12 0504Swedish Research Council, K2012-99X-21959-01-3 2006-5038-36531-16