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The Volatile Anesthetic Sevoflurane and Alterations in Neural Activity, as Measured by the Neural Regulated Protein ARC
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2015 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Background: General anesthesia, performed in surgery, reduces the neural activity. Sevoflurane is the most commonly used inhalation anesthetic in clinical. In the present study, the activity-regulated cytoskeleton-associated protein ARC is used to measure the alterations in neural activity from an exposure to sevoflurane. ARC is involved in the consolidation of long-term memory, and induced by exploration of novelty. It is unknown how long time it takes for the brain to recover to its baseline state of neuronal activity after a treatment with a general anesthetic.

Hypothesis: We hypothesize the neural activity to be decreased during the treatment, and increased above baseline after recovery.

Methods: Seventeen mice, divided in five groups, were studied and euthanized at different time points. The baseline group was euthanized prior to any treatment. The other groups either received sevoflurane or oxygen for three hours with half of the animals euthanized at the end of the treatment, and the other groups were allowed three hours recovery.

Results: A significant difference was shown between the anesthetized groups and the control groups when comparing the number of ARC expressing cells per unit area (P = 0.0472). There were no significant differences on the other measurements (P > 0.05), except for the mean intensity when comparing baseline and control group at the end of treatment (P = 0.0251). The neural activity in the anesthetized mice was decreased during the exposure, and this change persisted for at least 3 hours after recovery.

Conclusions: Sevoflurane decreases neural activity, as measured by the ARC protein in the dentate gyrus. Further studies are required to determine the duration of ARC expression depression after treatment with sevoflurane.

Place, publisher, year, edition, pages
2015. , 37 p.
Keyword [en]
General anesthesia, Sevoflurane
National Category
Other Natural Sciences Anesthesiology and Intensive Care Other Medical Sciences not elsewhere specified
URN: urn:nbn:se:uu:diva-275992OAI: oai:DiVA.org:uu-275992DiVA: diva2:901708
Subject / course
Pharmaceutical Pharmacology
Educational program
Master of Science Programme in Pharmacy
Available from: 2016-10-06 Created: 2016-02-08 Last updated: 2016-10-06Bibliographically approved

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