uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine. Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, S-75007 Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine. Riga Stradins Univ, August Kirchenstein Inst Microbiol & Virol, Riga, Latvia..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Virology.
Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, S-75007 Uppsala, Sweden.;Natl Food Adm Toxicol Lab, Dept Risk & Benefit Assessment, Uppsala, Sweden..
Show others and affiliations
2016 (English)In: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 60, no 1, 28-34 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

The high sequence variation of RNA viruses necessitates use of degenerate primers and probes or multiple primers and probes in molecular diagnostic assays. We showed previously that PCR amplification in two rounds, first with long target-specific primers and then with short generic primers, followed by detection using long probes, can tolerate sequence variation. Here we demonstrate that long primers and probes of up to 56 nucleotides can also be applied in real-time PCR for the detection of norovirus genogroup II with improved sensitivity. Probe design (method of incorporating quenchers, use of Zen internal quencher or traditional quenchers) greatly affects the sensitivity of the real-time PCR assays.

Place, publisher, year, edition, pages
2016. Vol. 60, no 1, 28-34 p.
Keyword [en]
real-time PCR, variation-tolerant capture multiplex assay, norovirus
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:uu:diva-276826DOI: 10.2144/000114370ISI: 000368058400003PubMedID: 26757809OAI: oai:DiVA.org:uu-276826DiVA: diva2:903493
Funder
EU, FP7, Seventh Framework Programme, 311846EU, FP7, Seventh Framework Programme, 316275
Available from: 2016-02-16 Created: 2016-02-16 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Öhrmalm, ChristinaBlomberg, Jonas

Search in DiVA

By author/editor
Öhrmalm, ChristinaBlomberg, Jonas
By organisation
Clinical Microbiology and Infectious MedicineClinical Virology
In the same journal
BioTechniques
Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 202 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf