uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Improving the Prediction of Prostate Cancer Overall Survival by Supplementing Readily Available Clinical Data with Gene Expression Levels of IGFBP3 and F3 in Formalin-Fixed Paraffin Embedded Core Needle Biopsy Material
Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden.;Chundsell Med AB, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Ridgeview Instruments AB, Uppsala, Sweden..
Karolinska Univ Hosp, Clin Pathol Cytol, Stockholm, Sweden..
Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden..
Show others and affiliations
2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 1, e0145545Article in journal (Refereed) PublishedText
Abstract [en]

Background A previously reported expression signature of three genes (IGFBP3, F3 and VGLL3) was shown to have potential prognostic value in estimating overall and cancer-specific survivals at diagnosis of prostate cancer in a pilot cohort study using freshly frozen Fine Needle Aspiration (FNA) samples. Methods We carried out a new cohort study with 241 prostate cancer patients diagnosed from 20042007 with a follow-up exceeding 6 years in order to verify the prognostic value of gene expression signature in formalin fixed paraffin embedded (FFPE) prostate core needle biopsy tissue samples. The cohort consisted of four patient groups with different survival times and death causes. A four multiplex one-step RT-qPCR test kit, designed and optimized for measuring the expression signature in FFPE core needle biopsy samples, was used. In archive FFPE biopsy samples the expression differences of two genes (IGFBP3 and F3) were measured. The survival time predictions using the current clinical parameters only, such as age at diagnosis, Gleason score, PSA value and tumor stage, and clinical parameters supplemented with the expression levels of IGFBP3 and F3, were compared. Results When combined with currently used clinical parameters, the gene expression levels of IGFBP3 and F3 are improving the prediction of survival time as compared to using clinical parameters alone. Conclusion The assessment of IGFBP3 and F3 gene expression levels in FFPE prostate cancer tissue would provide an improved survival prediction for prostate cancer patients at the time of diagnosis.

Place, publisher, year, edition, pages
2016. Vol. 11, no 1, e0145545
National Category
Cancer and Oncology
URN: urn:nbn:se:uu:diva-276821DOI: 10.1371/journal.pone.0145545ISI: 000367801400039PubMedID: 26731648OAI: oai:DiVA.org:uu-276821DiVA: diva2:903496
Swedish Cancer SocietyStockholm County Council
Available from: 2016-02-16 Created: 2016-02-16 Last updated: 2016-02-16Bibliographically approved

Open Access in DiVA

fulltext(1163 kB)48 downloads
File information
File name FULLTEXT01.pdfFile size 1163 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed
By organisation
Department of Immunology, Genetics and Pathology
In the same journal
Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar
Total: 48 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 57 hits
ReferencesLink to record
Permanent link

Direct link