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A comprehensive polymerase chain reaction-oligonucleotide typing system for the HLA class I A locus
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
1994 (English)In: Human Immunology, ISSN 0198-8859, E-ISSN 1879-1166, Vol. 40, no 1, 25-32 p.Article in journal (Refereed) Published
Abstract [en]

A comprehensive system for genetic typing of the HLA class I A locus is described, based on PCR amplification and typing with nonradioactively labeled SSO probes. Exons 1-3 of the A locus are amplified and typing is performed with a set of 30 nonradioactively labeled oligonucleotide probes. This system resolves 34 of 39 known alleles and 561 (94%) of 595 possible genotypes. Among a sample of 354 individuals from Sweden and China, 97.5% of the genotypes were resolved. Probes were directed preferentially at replacement substitutions in foreign antigen-binding sites, in order to detect not only the known alleles but also new combinations of polymorphic motifs, indicative of previously unrecognized alleles. Three individuals were found with a new combination of polymorphic motifs, suggesting the presence of at least one previously undescribed allele in the populations sampled. This typing system is useful for disease association studies, tissue typing, and in forensic medicine.

Place, publisher, year, edition, pages
1994. Vol. 40, no 1, 25-32 p.
National Category
Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-62803DOI: 10.1016/0198-8859(94)90018-3PubMedID: 8045790OAI: oai:DiVA.org:uu-62803DiVA: diva2:90714
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-11-30Bibliographically approved

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Allen, MarieGyllensten, Ulf

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