Cyclotides in the Violaceae
2015 (English)In: Plant Cyclotides / [ed] Craik, David J., Academic Press , 2015, Vol. 76, 15-49 p.Chapter in book (Other academic)Text
Abstract It has been 20 years since the cyclic cystine-knotted compounds, called cyclotides, tied our knot with the violets. This chapter, above all, is a lucid summary of the twists, turns, knots, and rings in the relationship that followed. The chapter begins by giving an account of how a little known scientific article facilitated the discovery of cyclotides in Violaceae, how it captured our imagination, and how it was realized that the discovery of a series of cyclotides was within a touching distance. The processes of extraction, isolation, and characterization as well as chemical synthesis of cyclotides used in our laboratory have been described, and in doing so, the solvents, materials, conditions, instrumentation, and techniques involved have been pointed out. An introduction to Violaceae and a description of these plants in diverse cultural settings for various therapeutic purposes is provided. A section follows it on the discovery of cycloviolacin O2 from Viola odorata as a potent antimicrobial peptide, and how its interaction with bacteria in terms of mechanism of action and resistance development was investigated. It is followed by a reflection on how the recent innovations in biotechnology and bioinformatics have helped out along the way: the use of novel strategies for cyclization and the use of transcriptomics data in the discovery of new cyclotides. Finally, the question about biological role of cyclotides in Violaceae has been asked, and an attempt to answer this question has been presented.
Place, publisher, year, edition, pages
Academic Press , 2015. Vol. 76, 15-49 p.
, Advances in Botanical Research, 76
Cyclotides, Discovery, Pharmacognosy, Traditional medicine, Violaceae
Research subject Pharmacognosy
IdentifiersURN: urn:nbn:se:uu:diva-278985DOI: 10.1016/bs.abr.2015.09.001ISI: 000376190900003ISBN: 9780128007976ISBN: 9780128000304OAI: oai:DiVA.org:uu-278985DiVA: diva2:907329