Successful enrichment of the ubiquitous freshwater acI Actinobacteria
2014 (English)In: Environmental Microbiology Reports, ISSN 1758-2229, E-ISSN 1758-2229, Vol. 6, no 1, 21-27 p.Article in journal (Refereed) PublishedText
Actinobacteriaof the acI lineage are often thenumerically dominant bacterial phylum in surfacefreshwaters, where they can account for>50% oftotal bacteria. Despite their abundance, there are nodescribed isolates. In an effort to obtain enrichmentof these ubiquitous freshwaterActinobacteria, dilutedfreshwater samples from Lake Grosse Fuchskuhle,Germany, were incubated in 96-well culture plates.With this method, a successful enrichment containinghigh abundances of a member of the lineage acI wasestablished. Phylogenetic classification showed thatthe acIActinobacteriaof the enrichment belonged tothe acI-B2 tribe, which seems to prefer acidic lakes.This enrichment grows to low cell densities and thusthe oligotrophic nature of acI-B2 was confirmed.
Place, publisher, year, edition, pages
2014. Vol. 6, no 1, 21-27 p.
IdentifiersURN: urn:nbn:se:uu:diva-279800DOI: 10. 1111/1758-2229.12104OAI: oai:DiVA.org:uu-279800DiVA: diva2:908953