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Rat model of chronic tympanic membrane perforation: Ventilation tube with mitomycin C and dexamethasone
Univ Western Australia, Sch Surg, Ear Sci Ctr, Perth, WA 6009, Australia.;Ear Sci Inst Australia, Perth, WA, Australia.;Sir Charles Gairdner Hosp, Skull Base Surg, Dept Otolaryngol Head & Neck, Perth, WA, Australia..
Univ Western Australia, Sch Surg, Ear Sci Ctr, Perth, WA 6009, Australia.;Ear Sci Inst Australia, Perth, WA, Australia.;Ningbo Lihuili Hosp, Ningbo Med Ctr, Dept Otolaryngol Head & Neck Surg, Ningbo, Zhejiang, Peoples R China..
Univ Western Australia, Sch Surg, Ear Sci Ctr, Perth, WA 6009, Australia.;Ear Sci Inst Australia, Perth, WA, Australia..
Univ Western Australia, Sch Surg, Ear Sci Ctr, Perth, WA 6009, Australia..
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2016 (English)In: International Journal of Pediatric Otorhinolaryngology, ISSN 0165-5876, E-ISSN 1872-8464, Vol. 80, no 1, 61-68 p.Article in journal (Refereed) Published
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Abstract [en]

Objective: Chronic tympanic membrane perforation (TMP) in a clinical setting may attract surgical intervention. With the advent of modern biomaterials, new options are available for myringoplasty but safety and efficacy need evaluation in a chronic TMP animal model. The aim of this study was to evaluate the efficacy of ventilation tube (VT) insertion in conjunction with topical application of mitomycin Cl dexamethasone (M/D) for the creation of chronic TMP in rats. Methods: Thirty male Sprague-Dawley rats underwent myringotomy of the right tympanic membrane (TM) and were divided into three experimental groups: spontaneous healing (myringotomy control), VT insertion for 2 weeks and VT insertion for 2 weeks in conjunction with topical application of M/D (VT-M/D). All TMs were regularly assessed by otoscopy for 10 weeks and then animals were sacrificed for histological evaluation. Results: In the VT-M/D group, seven out of ten (70%) perforations were patent at 10 weeks (mean patency, 57.9 days; P < 0.01). The VT group had two out of ten (20%) perforations patent at 10 weeks (mean patency, 26.5 days; P < 0.01), while all TMPs from the myringotomy control group were closed by day 9 (mean patency, 7.2 days). Histologically, the TMPs patent at week 10 had a stratified squamous epithelialized rim, keratinocyte layer thickening around the perforation edge as well as increased collagen deposition and macrophage infiltration. Conclusion: Chronic TMP in a rat model was successfully created by VT insertion and the efficacy was increased in combination with topical application of M/D.

Place, publisher, year, edition, pages
2016. Vol. 80, no 1, 61-68 p.
Keyword [en]
Tympanic membrane perforation, Chronic, Animal model, Ventilation tube, Mitomycin C, Dexamethasone
National Category
Otorhinolaryngology Pediatrics
Identifiers
URN: urn:nbn:se:uu:diva-280100DOI: 10.1016/j.ijporl.2015.11.010ISI: 000368951100013PubMedID: 26746614OAI: oai:DiVA.org:uu-280100DiVA: diva2:910121
Available from: 2016-03-08 Created: 2016-03-08 Last updated: 2017-11-30Bibliographically approved

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