Dynamic and selective HERV RNA expression in neuroblastoma cells subjected to variation in oxygen tension and demethylation
2016 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 1-2, 140-149 p.Article in journal (Refereed) PublishedText
We studied HERV expression in cell lines after hypoxia, mitogenic stimulation, and demethylation, to better understand if hypoxia may play a role in ERV activation also within the nervous system, as represented by neuroblastoma cell lines. The level of RNA of four human ERV groups (HERVs) (HERVE, I/T, H, and W), and three housekeeping genes, of different cell lines including A549, COS-1, Namalwa, RD-L and Vero-E6, as well as human neuroblastoma cell lines SH-SY5Y, SK-N-DZ, and SK-N-AS were studied using reverse transcription and real-time quantitative PCR (QPCR). During the course of recovery from hypoxia a pronounced and selective activation of RNA expression of HERVW-like sequences, but not of HERVE, I/T, H, and three housekeeping genes, was found in the neuroblastoma cell lines, most pronounced in SK-N-DZ. In the SK-N-DZ cell line, we also tested the expression of HERVs after chemical treatments. HERVW-like sequences were selectively upregulated by 5-azacytidine, a demethylating agent. Some HERVW loci seem especially responsive to hypoxia and demethylation. HERV expression in neuroblastoma cells is selectively and profoundly influenced by some physiological and chemical stimuli.
Place, publisher, year, edition, pages
2016. Vol. 124, no 1-2, 140-149 p.
Human endogenous retroviral sequences, neuroblastoma, hypoxia, RNA expression, Co-option, demethylation
Microbiology in the medical area Immunology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-280907DOI: 10.1111/apm.12494ISI: 000370081300013PubMedID: 26818268OAI: oai:DiVA.org:uu-280907DiVA: diva2:912313
FunderSwedish Research Council, K2004-32X-14252-03ASwedish Childhood Cancer Foundation