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Fast, rugged and sensitive ultra high pressure liquid chromatography tandem mass spectrometry method for analysis of cyanotoxins in raw water and drinking water-First findings of anatoxins, cylindrospermopsins and microcystin variants in Swedish source waters and infiltration ponds
Natl Food Agcy, Dept Sci, Div Chem, POB 622, S-75126 Uppsala, Sweden..
Natl Food Agcy, Dept Sci, Div Chem, POB 622, S-75126 Uppsala, Sweden..
Natl Food Agcy, Dept Sci, Div Chem, POB 622, S-75126 Uppsala, Sweden.;OnTarget Chem, Virdings Alle 32 B, S-75450 Uppsala, Sweden..
AS Tallinna Vesi, Jarvevana Tee 3, EE-10132 Tallinn, Estonia..
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2016 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1429, 265-276 p.Article in journal (Refereed) Published
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Abstract [en]

Freshwater blooms of cyanobacteria (blue-green algae) in source waters are generally composed of several different strains with the capability to produce a variety of toxins. The major exposure routes for humans are direct contact with recreational waters and ingestion of drinking water not efficiently treated. The ultra high pressure liquid chromatography tandem mass spectrometry based analytical method presented here allows simultaneous analysis of 22 cyanotoxins from different toxin groups, including anatoxins, cylindrospermopsins, nodularin and microcystins in raw water and drinking water. The use of reference standards enables correct identification of toxins as well as precision of the quantification and due to matrix effects, recovery correction is required. The multi-toxin group method presented here, does not compromise sensitivity, despite the large number of analytes. The limit of quantification was set to 0.1 mu g/L for 75% of the cyanotoxins in drinking water and 0.5 mu g/L for all cyanotoxins in raw water, which is compliant with the WHO guidance value for microcystin-LR. The matrix effects experienced during analysis were reasonable for most analytes, considering the large volume injected into the mass spectrometer. The time of analysis, including lysing of cell bound toxins, is less than three hours. Furthermore, the method was tested in Swedish source waters and infiltration ponds resulting in evidence of presence of anatoxin, homo-anatoxin, cylindrospermopsin and several variants of microcystins for the first time in Sweden, proving its usefulness.

Place, publisher, year, edition, pages
2016. Vol. 1429, 265-276 p.
Keyword [en]
UPLC-MS/MS, Cell lysing, Cyanotoxin, Drinking water, Blue-green algae, Cyanobacteria
National Category
Biochemistry and Molecular Biology Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-280900DOI: 10.1016/j.chroma.2015.12.049ISI: 000369559100029PubMedID: 26755412OAI: oai:DiVA.org:uu-280900DiVA: diva2:912328
Funder
Swedish Civil Contingencies Agency, SOFA 24-12
Available from: 2016-03-16 Created: 2016-03-16 Last updated: 2017-11-30Bibliographically approved

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Sundström, L. Fredrik

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