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Tissue sample stability: thawing effect on multi-organ samples
Umea Univ, Dept Chem, Computat Life Sci Cluster CLiC, Umea, Sweden.;Karlsruhe Inst Technol, D-76021 Karlsruhe, Germany..
AcureOm AB, Umea, Sweden..
CNRS, UMR7622, F-75005 Paris, France.;Univ Paris 04, UPMC, UMR7622, F-75005 Paris, France.;INSERM, U1156, Paris, France..
AcureOm AB, Umea, Sweden..
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2016 (English)In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 12, no 2, 19Article in journal (Refereed) PublishedText
Abstract [en]

Correct handling of samples is essential in metabolomic studies. Improper handling and prolonged storage of samples has unwanted effects on the metabolite levels. The aim of this study was to identify the effects that thawing has on different organ samples. Organ samples from gut, kidney, liver, muscle and pancreas were analyzed for a number of endogenous metabolites in an untargeted metabolomics approach, using gas chromatography time of flight mass spectrometry at the Swedish Metabolomics Centre, Umeao University, Sweden. Multivariate data analysis was performed by means of principal component analysis and orthogonal projection to latent structures discriminant analysis. The results showed that the metabolic changes caused by thawing were almost identical for all organs. As expected, there was a marked increase in overall metabolite levels after thawing, caused by increased protein and cell degradation. Cholesterol was one of the eight metabolites found to be decreased in the thawed samples in all organ groups. The results also indicated that the muscles are less susceptible to oxidation compared to the rest of the organ samples.

Place, publisher, year, edition, pages
2016. Vol. 12, no 2, 19
Keyword [en]
Thawing effect, Metabolomics, OPLS, Multivariate analysis, Multi-organ
National Category
Endocrinology and Diabetes
URN: urn:nbn:se:uu:diva-280892DOI: 10.1007/s11306-015-0933-1ISI: 000369343900010OAI: oai:DiVA.org:uu-280892DiVA: diva2:912352
Swedish Research Council, 2011-6044
Available from: 2016-03-16 Created: 2016-03-16 Last updated: 2016-04-01Bibliographically approved

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