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Nanocrystalline diamond sensor targeted for selective CRP detection: An ATR-FTIR spectroscopy study
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. FOI Swedish Def Res Agcy, CBRN Def & Secur, S-90182 Umea, Sweden.; Mol Fingerprint Sweden AB, Eksatravagen 130, S-75655 Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. Mol Fingerprint Sweden AB, Eksatravagen 130, S-75655 Uppsala, Sweden.
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2016 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 408, no 14, 3675-3680 p.Article in journal (Refereed) Published
Abstract [en]

Protein immobilization on functionalized fluorine- terminated nanocrystalline (NCD) films was studied by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using an immobilization protocol developed to specifically bind C-reactive protein (CRP). Using an ATR- FTIR spectroscopy method employing a force-controlled anvil-type configuration, three critical steps of the ex situ CRP immobilization were analyzed. First, the NCD surface was passivated by deposition of a copolymer layer consisting of polyethylene oxide and polypropylene oxide. Second, a synthetic modified polypeptide binder with high affinity to CRP was covalently attached to the polymeric film. Third, CRP dissolved in aqueous buffer in concentrations of 10–20 μg/ mL was added on the functionalized NCD surface. Both the amide I and II bands, due to the polypeptide binder and CRP, were clearly observed in ATR-FTIR spectra. CRP amide I bands were extracted from difference spectra and yielded bands that agreed well with the reported amide I band of free (non-bonded) CRP in solution. Thus, our results show that CRP retains its secondary structure when it is attached to the polypeptide binders. Compared to previous IR studies of CRP in solution, about 200 times lower concentration was applied in the present study. 

Place, publisher, year, edition, pages
2016. Vol. 408, no 14, 3675-3680 p.
National Category
Analytical Chemistry Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-281044DOI: 10.1007/s00216-016-9485-0ISI: 000375057400001OAI: oai:DiVA.org:uu-281044DiVA: diva2:912583
Funder
Swedish Research Council, 621-2014-5959
Available from: 2016-03-16 Created: 2016-03-16 Last updated: 2017-11-30Bibliographically approved

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Andersson, Per OlaForsberg, PontusNikolajeff, FredrikÖsterlund, LarsKarlsson, Mikael

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