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Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lanekoff group)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lanekoff group)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lanekoff group)
2016 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 141, no 12, 3686-3695 p.Article in journal (Refereed) Published
Abstract [en]

Small molecule neurotransmitters are essential for the function of the nervous system, and neurotransmitter imbalances are often connected to neurological disorders. The ability to quantify such imbalances is important to provide insights into the biochemical mechanisms underlying the disorder. This proof-of-principle study presents online quantification of small molecule neurotransmitters, specifically acetylcholine, γ-aminobutyric acid (GABA) and glutamate, in rat brain tissue sections using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging. By incorporating deuterated internal standards in the nano-DESI solvent we show identification, accurate mapping, and quantification of these small neurotransmitters in rat brain tissue without introducing any additional sample preparation steps. We find that GABA is about twice as abundant in the medial septum-diagonal band complex (MSDB) as in the cortex, while glutamate is about twice as abundant in the cortex as compared to the MSDB. The study shows that nano-DESI is well suited for imaging of small molecule neurotransmitters in health and disease.

Place, publisher, year, edition, pages
2016. Vol. 141, no 12, 3686-3695 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-281314DOI: 10.1039/c5an02620bISI: 000378942900021PubMedID: 26859000OAI: oai:DiVA.org:uu-281314DiVA: diva2:913788
Funder
Swedish Research Council, VR 621-2013-4231Swedish Foundation for Strategic Research , SSF ICA-6
Available from: 2016-03-22 Created: 2016-03-22 Last updated: 2017-11-30Bibliographically approved
In thesis
1. Applications of nanospray desorption electrospray ionization mass spectrome: Analysis of lipids and metabolites in brain tissue sections and single cells
Open this publication in new window or tab >>Applications of nanospray desorption electrospray ionization mass spectrome: Analysis of lipids and metabolites in brain tissue sections and single cells
2016 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Uppsala: Department of Chemistry, Uppsala University, 2016. 37 p.
Keyword
Nano-DESI, single cell analysis, small molecule neurotransmitters, mass spectrometry imaging, MSI, GABA, Glutamate, Acetylcholine
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-291339 (URN)
Presentation
2016-05-31, B7:113a, Husargatan 3, Uppsala, 10:15 (English)
Opponent
Supervisors
Available from: 2016-11-28 Created: 2016-05-01 Last updated: 2016-11-28Bibliographically approved

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