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Bioremediation optimization of diclofenac using Trametes versicolor with emphasis on analytical pharmaceutical chemistry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Diclofenac has been reported to give adverse effects to water-living organisms at

environmentally relevant concentrations and the removal rate of this substance in

sewage treatment plants is poor, and therefor other techniques needs to be

examined. The purpose of this study was to evaluate the ability of the white rot

fungus Trametes versicolor to degrade diclofenac. An enzymatic assay was developed,

where the activity of laccase was monitored. The concentration of diclofenac in

E-flask and bioreactor experiments was measured using UHPLC-Q-Tof with a C18

column. Three controls were used to fully investigate the observed diclofenac

concentration decrease. Fungal mycelia were grown in nutrient solution and

immobilized on polyurethane carriers. The initial concentration of diclofenac was 10

mg/L and samples were taken at determined intervals during one week. The results

showed a fast decrease in diclofenac concentration, and a clear difference could be

seen between the active culture and the controls in the E-flask experiments. The

E-flask experiments also had a high enzymatic activity throughout the week, while the

enzymatic activity exponentially decreased in the bioreactor experiment. The

enzymatic activity decrease corresponded well with the diclofenac concentration in

the bioreactor, which was lower than the controls within the first four hours, and

thereafter increased to the same level as the controls. Four degradation products of

diclofenac were found in the E-flask experiments and the fungi was also capable to

degrade other aromatic substances that originated from the carrier material.

Place, publisher, year, edition, pages
2016. , 29 p.
Series
UPTEC K, ISSN 1650-8297 ; 15038
National Category
Other Chemical Engineering
Identifiers
URN: urn:nbn:se:uu:diva-281350OAI: oai:DiVA.org:uu-281350DiVA: diva2:914408
External cooperation
General Electric Healthcare
Educational program
Master Programme in Chemical Engineering
Supervisors
Examiners
Available from: 2016-07-05 Created: 2016-03-22 Last updated: 2016-07-05Bibliographically approved

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