uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
No outgrowth of chondrocytes from non-digested particulated articular cartilage embedded in commercially available fibrin matrix: an in vitro study
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Clinical Research, County of Västmanland. Vastmanland Cty Hosp, Dept Orthopaed, Vasteras, Sweden..
Vastmanland Cty Hosp, Dept Pathol, Vasteras, Sweden..
Vastmanland Cty Hosp, Dept Clin Chem, Vasteras, Sweden..
2016 (English)In: Journal of Orthopaedic Surgery and Research, ISSN 1749-799X, E-ISSN 1749-799X, Vol. 11, 23Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Background: Commercially available fibrin is routinely being used as both a matrix in certain cartilage repair techniques and a method for scaffold fixation. Chondrocytes from non-digested particulated cartilage fragments are proposed as a possible source for new cartilage tissue formation in some operative techniques. The goal of this study was to test that chondrocytes from particulated articular cartilage embedded in fibrin have an active role in the process of cartilage repair, as well as if commercially available fibrin should be used as a suitable matrix. Methods: Articular cartilage was obtained from patients undergoing total knee replacement surgery. The biopsies were particulated in small, 1-2-mm(3) pieces and embedded in fibrin. Two groups were compared in our study, particulated articular cartilage with and without collagenase treatment. The specimens were analyzed by optical microscopy after 2-5 weeks of cultivation in a special construct embedded in a cell culture medium containing particulated cartilage embedded in fibrin in the upper phase and cancellous bone in the lower phase under the perforated nylon membrane. Results: None of the biopsies taken from four different patients showed the outgrowth of chondrocytes or bone marrow-originated cells into the fibrin matrix in our experimental model. Conclusions: It has been shown in our experimental model in vitro little to support the theory that articular chondrocytes from particulated articular cartilage embedded in fibrin have an active role in cartilage repair in its early stage.

Place, publisher, year, edition, pages
2016. Vol. 11, 23
Keyword [en]
Articular chondrocytes, Particulated cartilage, Fibrin, Technique
National Category
Orthopedics
Identifiers
URN: urn:nbn:se:uu:diva-281802DOI: 10.1186/s13018-016-0355-4ISI: 000370242600001PubMedID: 26879178OAI: oai:DiVA.org:uu-281802DiVA: diva2:915667
Available from: 2016-03-30 Created: 2016-03-30 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

fulltext(1183 kB)57 downloads
File information
File name FULLTEXT01.pdfFile size 1183 kBChecksum SHA-512
c1d6cd66a97fb3849245ea4bad9d5fc0a0d45905f41bb2aa6a7f55424eb99dbcfa1b9b07113c4d86d9ef66fd40ed0c40a3d41e990f5a602d5cfd09c0b6590691
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed
By organisation
Centre for Clinical Research, County of Västmanland
In the same journal
Journal of Orthopaedic Surgery and Research
Orthopedics

Search outside of DiVA

GoogleGoogle Scholar
Total: 57 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 229 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf