Development of a chiral non-aqueous capillary electrophoretic system using the partial filling technique with UV and mass spectrometric detection
2003 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 986, no 1, 143-152 p.Article in journal (Refereed) Published
A chiral non-aqueous CE system with UV and mass spectrometric detection has been developed. The enantioseparation was promoted by diastereomeric complex (ion-pair) formation between the amines (e.g. salbutamol, atenolol) and the chiral selector, (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid [(-)-DIKGA]. Different solvent mixtures were studied, as well as different concentrations of (-)-DIKGA and ammonium acetate in the background electrolyte. A partial filling technique was developed with a selector plug composed of (-)-DIKGA and ammonium acetate in a solvent mixture of methanol and 2-propanol. The separated enantiomers of pronethalol were detected by a Q-TOF MS system equipped with a sheath-flow electrospray ionization interface.
Place, publisher, year, edition, pages
Elsevier, 2003. Vol. 986, no 1, 143-152 p.
Non-aqueous capillary electrophoresis; Enantiomer separation; Partial filling technique; Amines; (2)-DIKGA; Pronethalol
Analytical Chemistry Pharmaceutical Sciences
IdentifiersURN: urn:nbn:se:uu:diva-63662DOI: 10.1016/S0021-9673(02)01866-6PubMedID: 12585332OAI: oai:DiVA.org:uu-63662DiVA: diva2:91573