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Dynamic niche-specific adaptations in Neisseria meningitidis during infection
Inner Mongolia Univ Nationalities, Sch Life Sci, Tongliao 028000, Peoples R China..
Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, S-10691 Stockholm, Sweden.;Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Peoples R China.;Kitasato Univ, Grad Sch Infect Control Sci, Tokyo 1088641, Japan..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, S-10691 Stockholm, Sweden..
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2016 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 18, no 2, 109-117 p.Article in journal (Refereed) PublishedText
Abstract [en]

Neisseria meningitidis is an opportunistic human pathogen that usually colonizes the nasopharyngeal mucosa asymptomatically. Upon invasion into the blood and central nervous system, this bacterium triggers a fulminant inflammatory reaction with the manifestations of septicemia and meningitis, causing high morbidity and mortality. To reveal the bacterial adaptations to specific and dynamic host environments, we performed a comprehensive proteomic survey of N. meningitidis isolated from the nasal mucosa, CSF and blood of a mouse disease model. We could identify 51 proteins whose expression pattern has been changed during infection, many of which have not yet been characterized. The abundance of proteins was markedly lower in the bacteria isolated from the nasal mucosa compared to the bacteria from the blood and CSF, indicating that initiating adhesion is the harshest challenge for meningococci. The high abundance of the glutamate dehydrogenase (GdhA) and Opa1800 proteins in all bacterial isolates suggests their essential role in bacterial survival in vivo. To evaluate the biological relevance of our proteomic findings, four candidate proteins from representative functional groups, such as the bacterial chaperone GroEL, IMP dehydrogenase GuaB, and membrane proteins PilQ and NMC0101, were selected and their impact on bacterial fitness was investigated by mutagenesis assays. This study provides an integrated picture of bacterial niche-specific adaptations during consecutive infection processes.

Place, publisher, year, edition, pages
2016. Vol. 18, no 2, 109-117 p.
Keyword [en]
Neisseria meningitidis, Host adaptation, Virulence, Proteomic analysis
National Category
Infectious Medicine Immunology in the medical area
URN: urn:nbn:se:uu:diva-281966DOI: 10.1016/j.micinf.2015.09.025ISI: 000370375400005PubMedID: 26482500OAI: oai:DiVA.org:uu-281966DiVA: diva2:916221
Swedish Research Council, 2008-2572Swedish Research Council, 2008-3367
Available from: 2016-04-01 Created: 2016-04-01 Last updated: 2016-04-01Bibliographically approved

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