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Novel actin filaments from Bacillus thuringiensis form nanotubules for plasmid DNA segregation
Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore 138673, Singapore..
Nagoya Univ, Grad Sch Sci, Struct Biol Res Ctr, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648602, Japan.;Nagoya Univ, Grad Sch Sci, Struct Biol Res Ctr, Div Biol Sci,Chikusa Ku, Furo Cho, Nagoya, Aichi 4648602, Japan.;Div Biol Sci, Furo Cho, Nagoya, Aichi 4648602, Japan.;Japan Sci & Technol Agcy, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan..
Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore 138673, Singapore..
Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore 138673, Singapore..
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2016 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, no 9, E1200-E1205 p.Article in journal (Refereed) Published
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Abstract [en]

Here we report the discovery of a bacterial DNA-segregating actin-like protein (BtParM) from Bacillus thuringiensis, which forms novel antiparallel, two-stranded, supercoiled, nonpolar helical filaments, as determined by electron microscopy. The BtParM filament features of supercoiling and forming antiparallel double-strands are unique within the actin fold superfamily, and entirely different to the straight, double-stranded, polar helical filaments of all other known ParMs and of eukaryotic F-actin. The BtParM polymers show dynamic assembly and subsequent disassembly in the presence of ATP. BtParR, the DNA-BtParM linking protein, stimulated ATP hydrolysis/phosphate release by BtParM and paired two supercoiled BtParM filaments to form a cylinder, comprised of four strands with inner and outer diameters of 57 angstrom and 145 angstrom, respectively. Thus, in this prokaryote, the actin fold has evolved to produce a filament system with comparable features to the eukaryotic chromosome-segregating microtubule.

Place, publisher, year, edition, pages
2016. Vol. 113, no 9, E1200-E1205 p.
Keyword [en]
actin, ParM, plasmid, filament, microtubule
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-282309DOI: 10.1073/pnas.1600129113ISI: 000371204500012PubMedID: 26873105OAI: oai:DiVA.org:uu-282309DiVA: diva2:916862
Available from: 2016-04-05 Created: 2016-04-05 Last updated: 2017-11-30Bibliographically approved

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Larsson, Mårten

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