uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Zip14 expression induced by lipopolysaccharides in macrophages attenuates inflammatory response
Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
2013 (English)In: Inflammation Research, ISSN 1023-3830, E-ISSN 1420-908X, Vol. 62, no 2Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE AND DESIGN: We investigated the role and regulation of zinc transporters in the activation of the inflammatory response in macrophages. Our exploratory computational study found that Zip14 (SLC39A14) was consistently up-regulated in activated macrophages; we therefore focused subsequently on that gene in the mechanistic study.

MATERIAL: The expression and function of Zip14 was assessed in primary macrophages obtained by in-vitro differentiation of monocytes from human blood.

METHODS: Primary macrophages were subjected to treatments with lipopolysaccharides, cytokines, chemicals, and pharmacological agents. SLC39A14 and inflammatory cytokine gene expressions were assessed by RT-qPCR. Zip14 siRNA knockdown was performed to explore the gene function.

RESULTS: Lipopolysaccharide's inflammatory stimulus was a strong inducer of SLC39A14 mRNA expression in macrophages. This induction was dependent on calcium signaling, GC-rich DNA-binding, and NF-κB down-regulation. Impregnation of lipopolysaccharide-stimulated macrophages with the glucocorticoid dexamethasone further enhanced Zip14 expression while reducing interleukin-6 and tumor necrosis factor-α production. Zip14 knockdown in macrophages attenuated the expression and secretion of cytokines, indicating a buffering function for this zinc transporter.

CONCLUSIONS: Collectively, our results identified the zinc transporter Zip14 as expressed downstream of lipopolysaccharide signals in macrophages. Zip14 induction had a regulatory function in cytokine production.

Place, publisher, year, edition, pages
2013. Vol. 62, no 2
National Category
Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:uu:diva-282511DOI: 10.1007/s00011-012-0559-yPubMedID: 23052185OAI: oai:DiVA.org:uu-282511DiVA: diva2:917132
Available from: 2016-04-05 Created: 2016-04-05 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Sayadi, Ahmed

Search in DiVA

By author/editor
Sayadi, Ahmed
In the same journal
Inflammation Research
Rheumatology and Autoimmunity

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 176 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf