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Generation of human acute lymphoblastic leukemia xenografts for use in oncology drug discovery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology. (Holmfeldt)ORCID iD: 0000-0003-4140-3423
2015 (English)In: Current protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.], ISSN 1934-8290, Vol. 68Article in journal (Refereed) Published
Abstract [en]

The establishment of reproducible mouse models of acute lymphoblastic leukemia (ALL) is necessary to provide in vivo therapeutic test systems that recapitulate human ALL, and for amplification of limited amounts of primary tumor material. A popular assay is the primary xenograft model that utilizes immunocompromised mice. The protocol includes injection of primary patient tumor specimens into mice with subsequent serial passaging of the tumors by retransplants of cells harvested from the mouse bone marrow and spleen. The tumors generated are then used for genomic profiling, ex vivo compound testing, mechanistic studies and retransplantation. Detailed in this unit are procedures for the establishment and maintenance of primary ALL xenograft panels for use in basic research and translational studies.

Place, publisher, year, edition, pages
2015. Vol. 68
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Other Basic Medicine
URN: urn:nbn:se:uu:diva-282779DOI: 10.1002/0471141755.ph1432s68PubMedID: 25737157OAI: oai:DiVA.org:uu-282779DiVA: diva2:917525
Available from: 2016-04-06 Created: 2016-04-06 Last updated: 2016-04-06

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