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Identification of meat products by shotgun spectral matching
Leiden Univ, Med Ctr, Ctr Prote & Metab, Albinusdreef 2, NL-2300 ZC Leiden, Netherlands..
Leiden Univ, Med Ctr, Ctr Prote & Metab, Albinusdreef 2, NL-2300 ZC Leiden, Netherlands..
Leiden Univ, Med Ctr, Ctr Prote & Metab, Albinusdreef 2, NL-2300 ZC Leiden, Netherlands..
Novo Nordisk Fdn, Ctr Biosustainabil, DTU Biosustain, Kogle Alle 6, DK-2970 Horsholm, Denmark..
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2016 (English)In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 203, 28-34 p.Article in journal (Refereed) Published
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Text
Abstract [en]

A new method, based on shotgun spectral matching of peptide tandem mass spectra, was successfully applied to the identification of different food species. The method was demonstrated to work on raw as well as processed samples from 16 mammalian and 10 bird species by counting spectral matches to spectral libraries in a reference database with one spectral library per species. A phylogenetic tree could also be constructed directly from the spectra. Nearly all samples could be correctly identified at the species level, and 100% at the genus level. The method does not use any genomic information and unlike targeted methods, no prior knowledge of genetic variation within a genus or species is necessary.

Place, publisher, year, edition, pages
2016. Vol. 203, 28-34 p.
Keyword [en]
Food products, Shotgun proteomics, Identification, Spectral libraries, Quantitation
National Category
Food Science
Identifiers
URN: urn:nbn:se:uu:diva-282783DOI: 10.1016/j.foodchem.2016.01.138ISI: 000371393900005PubMedID: 26948585OAI: oai:DiVA.org:uu-282783DiVA: diva2:919214
Funder
Swedish Research Council, 621-2011-4423
Available from: 2016-04-13 Created: 2016-04-07 Last updated: 2017-11-30Bibliographically approved

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Bergquist, Jonas

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