Reduced oxidative stress in primary human cells by antioxidant released from nanoporous alumina
2016 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 104, no 3, 568-575 p.Article in journal (Refereed) Published
Nanoporous alumina elicits different inflammatory responses dependent on pore size, such as increased complement activation and reactive oxygen species (ROS) production, on 200 versus 20 nm pores. In this study, we attempt to further modulate inflammatory cell response by loading nanoporous alumina membranes (20, 100, and 200 nm pores), with an antioxidant, Trolox, for controlled drug release. For mononuclear cells (MNC) no difference in cell response, due to pore size, was seen when cultured on nonloaded membranes. However, when exposed to membranes loaded with Trolox, 100 uM was enough to quench ROS by more than 95% for all pore sizes. Polymorphonuclear cells (PMNC) produced significantly more ROS when exposed to 20 versus 100 nm pores. For Trolox loaded membranes, this trend reversed, due to slower release of antioxidant from the 20 nm pores. Furthermore, Trolox exhibited a unique effect on PMNCs that has not previously been reported: It delayed the production of ROS in a manner distinct from antioxidant activity. The present study confirms that nanoporous alumina is a suitable vehicle for drug delivery, and that Trolox can successfully modulate the inflammatory response of both MNC and PMNCs.
Place, publisher, year, edition, pages
2016. Vol. 104, no 3, 568-575 p.
nanoporous alumina; Trolox; mononuclear cells; polymorphonuclear cells; reactive oxygen species
Immunology Biomaterials Science Engineering and Technology
IdentifiersURN: urn:nbn:se:uu:diva-283488DOI: 10.1002/jbm.b.33427ISI: 000372297100016PubMedID: 25952986OAI: oai:DiVA.org:uu-283488DiVA: diva2:919298
FunderThe Swedish Foundation for International Cooperation in Research and Higher Education (STINT)