Stepwise nucleosome translocation by RSC remodeling complexes
2016 (English)In: eLIFE, E-ISSN 2050-084X, Vol. 5, e10051Article in journal (Refereed) PublishedText
Harada, Bryan T.
Harvard Univ, Grad Program Biophys, Cambridge, MA 02138 USA.;Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA..
Hwang, William L.
Harvard Univ, Grad Program Biophys, Cambridge, MA 02138 USA.;Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA.;Harvard Univ, Sch Med, Harvard MIT MD PhD Program, Boston, MA USA..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA.;Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA..
Univ Texas MD Anderson Canc Ctr, Dept Epigenet & Mol Carcinogenesis, Smithville, TX USA.;Univ Calif San Francisco, Sch Med, San Francisco, CA USA..
The SWI/SNF-family remodelers regulate chromatin structure by coupling the free energy from ATP hydrolysis to the repositioning and restructuring of nucleosomes, but how the ATPase activity of these enzymes drives the motion of DNA across the nucleosome remains unclear. Here, we used single-molecule FRET to monitor the remodeling of mononucleosomes by the yeast SWI/SNF remodeler, RSC. We observed that RSC primarily translocates DNA around the nucleosome without substantial displacement of the H2A-H2B dimer. At the sites where DNA enters and exits the nucleosome, the DNA moves largely along or near its canonical wrapping path. The translocation of DNA occurs in a stepwise manner, and at both sites where DNA enters and exits the nucleosome, the step size distributions exhibit a peak at approximately 1-2 bp. These results suggest that the movement of DNA across the nucleosome is likely coupled directly to DNA translocation by the ATPase at its binding site inside the nucleosome.
Place, publisher, year, edition, pages
2016. Vol. 5, e10051
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-283665DOI: 10.7554/eLife.10051ISI: 000371879300001OAI: oai:DiVA.org:uu-283665DiVA: diva2:919520