On tailoring and validating the pore size distribution of nanocellulose based virus removal filter
2016 (English)Conference paper, Poster (Other academic)
This work explores the possibility of controlling the pore size distribution of the nanocellulose based virus removal membrane by altering its manufacturing conditions. Further, the capacity of the nanocellulose filter toward surrogate 20 and 50 nm Au NPs is validated quantitatively. The nanocellulose-based membrane is a non-woven, µm-thick filter paper with a narrow and tailorable pore size distribution, highly suitable for virus removal as previously shown for large 80-100 nm viruses, e.g. swine influenza virus (SIV) and xenotropic murine leukemia virus (xMULV).
It is produced by hot-pressing method rather than by phase-inversion which is otherwise used to manufacture industrial analogues. Robust and cost-efficient virus removal processes are in high demand for the biotechnology industry, especially for the production of monoclonal antibodies derived from mammalian cell lines, therapeutic proteins derived from human plasma, and cell culture media. By physically removing virus particles with size exclusion filters, viruses can be selectively separated from proteins, and thus the risk of infection can be mitigated. The presented work is part of continuous efforts to develop a new type of paper filter that can also remove the smallest and most resistant to inactivation viruses, such as parvovirus, featuring a typical particle size around 20 nm. The work summarizes the results from a number of characterisations including e.g. SEM images of the membrane pre- and post filtration of surrogate gold Au NPs as well as BJH gas desorption analysis of data for pore size distribution.
Place, publisher, year, edition, pages
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-284414OAI: oai:DiVA.org:uu-284414DiVA: diva2:920324
American Chemical Society 2016 San Diego
FunderKnut and Alice Wallenberg Foundation