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Small, novel proteins from the mistletoe Phoradendron tomentosum exhibit highly selective cytotoxicity to human breast cancer cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Cancer Pharmacology and Informatics)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
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2003 (English)In: Cellular and Molecular Life Sciences (CMLS), ISSN 1420-682X, E-ISSN 1420-9071, Vol. 60, no 1, 165-175 p.Article in journal (Refereed) Published
Abstract [en]

Four novel proteins (phoratoxins C-F) have been isolated from the North American mistletoe Phoradendron tomentosum. The amino acid sequences of these phoratoxins were determined unambiguously using a combination of Edman degradation and trypsin enzymatic digestion, and by electrospray ionization tandem mass spectrometry sequencing. Phoratoxins C, E and F consist of 46 amino acid residues; and phoratoxin D of 41. All proteins had six cysteines, similar to the earlier described phoratoxins A and B, which are thionins. The cytotoxicity of each protein was evaluated in a human cell line panel that represented several cytotoxic drug-resistance mechanisms. For the half-maximal inhibitory concentrations (IC50 values) of the different cell lines in the panel, correlation with those of standard drugs was low. The most potent cytotoxic phoratoxin C was further tested on primary cultures of human tumor cells from patients. The solid tumor samples from breast cancer cells were 18 times more sensitive to phoratoxin C than the tested hematological tumor samples.

Place, publisher, year, edition, pages
2003. Vol. 60, no 1, 165-175 p.
Keyword [en]
Breast cancer, cytotoxicity drug screening, mistletoe proteins, Phoradendron tomentosum, phoratoxins, thionins, tumor cell lines
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-64178DOI: 10.1007/s000180300011PubMedID: 12613665OAI: oai:DiVA.org:uu-64178DiVA: diva2:92089
Available from: 2007-02-08 Created: 2007-02-08 Last updated: 2017-11-30Bibliographically approved

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Gullbo, JoachimLarsson, Rolf

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