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Evaluation of the Impact of Different Visualization Techniques on DNA in Fingerprints
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik.
2013 (English)In: Journal of Forensic Identification, ISSN 1556-4029, Vol. 63, no 2, 189-204 p.Article in journal (Refereed) Published
Abstract [en]

More than 200 latent fingerprints were deposited on various surfaces under controlled conditions and then developed using nine different visualization techniques. DNA was extracted from the fingerprints and the samples were subsequently quantified for nuclear and mitochondrial DNA content, using real-time PCR. The results show that several of the evaluated visualization techniques (e.g., Wet Powder and black fingerprint powder) do not damage DNA and allow DNA analysis to a large extent. However, some of the visualization techniques (e.g., physical developer and silver nitrate) seem to eliminate DNA completely, highly degrade DNA, or introduce inhibitors, preventing subsequent analysis. Furthermore, the results demonstrate great variation in DNA amounts detected in samples developed with the same method.

Place, publisher, year, edition, pages
2013. Vol. 63, no 2, 189-204 p.
National Category
Forensic Science
Identifiers
URN: urn:nbn:se:uu:diva-285836OAI: oai:DiVA.org:uu-285836DiVA: diva2:921206
Available from: 2016-04-19 Created: 2016-04-19 Last updated: 2017-08-29Bibliographically approved

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Allen, Marie

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