Direct astatination of a tumour-binding protein, human Epidermal Growth Factor, using nido-carborane as a prosthetic group
2003 (English)In: Journal of Radioanalytical and Nuclear Chemistry, ISSN 0236-5731, Vol. 256, no 2, 191-197 p.Article in journal (Refereed) Published
We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68±9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate.
Place, publisher, year, edition, pages
2003. Vol. 256, no 2, 191-197 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-64296DOI: 10.1023/A:1023912728929OAI: oai:DiVA.org:uu-64296DiVA: diva2:92207