Radio-iodination of monoclonal antibody using potassium [125I]-(4-isothiocyanatobenzylammonio)-iodo-decahydro-closo-dodecaborate (iodo-DABI)
2006 (English)In: Anticancer Research, ISSN 0250-7005, Vol. 26, no 2A, 1217-1223 p.Article, book review (Other academic) Published
Negatively-charged polyhedral boron clusters can be easily halogenated with highly stable boron-halogen bonds and are promising in radionuclide diagnostics and cancer therapy.
MATERIALS AND METHODS:
The radio-iodination conditions for the closo-dodecaborate anion and for the conjugation of its labeled isothiocyanatobenzylammonio derivative to the monoclonal antibody (mAb) were optimized.
The labeling yield was about 90% and the overall conjugation yield was 55-60%. The in vitro stability of the radio-iodinated mAb was good under physiological and non-physiological conditions. The immunoreactivity of the labeled mAb (SK-BR-3 cells) was retained in the one-pot two-step labeling.
Negatively-charged polyhedral boron clusters can be used for indirect radio-iodination of mAbs.
Place, publisher, year, edition, pages
2006. Vol. 26, no 2A, 1217-1223 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-64297PubMedID: 16619527OAI: oai:DiVA.org:uu-64297DiVA: diva2:92208