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Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study
Univ Oslo, Inst Basic Med Sci, Dept Nutr, POB 1046, N-0317 Oslo, Norway..
Univ Eastern Finland, Inst Publ Hlth & Clin Nutr, Kuopio, Finland..
Univ Oslo, Inst Basic Med Sci, Dept Nutr, POB 1046, N-0317 Oslo, Norway..
Karolinska Inst, Dept Med H7, Stockholm, Sweden..
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2016 (English)In: Genes & Nutrition, ISSN 1555-8932, E-ISSN 1865-3499, Vol. 11, no 1, 3Article in journal (Refereed) PublishedText
Abstract [en]

Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of +/- 4 kg, high-sensitivity C-reactive protein concentration <= 10 mg L-1, and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

Place, publisher, year, edition, pages
2016. Vol. 11, no 1, 3
Keyword [en]
mRNA gene expression, Metabolic syndrome, PBMCs, Nordic diet, OGTT
National Category
Health Sciences
URN: urn:nbn:se:uu:diva-286658DOI: 10.1186/s12263-016-0521-4ISI: 000372581000003OAI: oai:DiVA.org:uu-286658DiVA: diva2:923693
Swedish Research CouncilSwedish Diabetes AssociationSwedish Heart Lung Foundation
Available from: 2016-04-27 Created: 2016-04-21 Last updated: 2016-04-27Bibliographically approved

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Rosqvist, FredrikRiserus, Ulf
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