The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing
2016 (English)In: Nature Immunology, ISSN 1529-2908, E-ISSN 1529-2916, Vol. 17, no 4, 451-460 p.Article in journal (Refereed) PublishedText
Innate lymphoid cells (ILCs) are increasingly appreciated as important participants in homeostasis and inflammation. Substantial plasticity and heterogeneity among ILC populations have been reported. Here we have delineated the heterogeneity of human ILCs through single-cell RNA sequencing of several hundreds of individual tonsil CD127(+) ILCs and natural killer (NK) cells. Unbiased transcriptional clustering revealed four distinct populations, corresponding to ILC1 cells, ILC2 cells, ILC3 cells and NK cells, with their respective transcriptomes recapitulating known as well as unknown transcriptional profiles. The single-cell resolution additionally divulged three transcriptionally and functionally diverse subpopulations of ILC3 cells. Our systematic comparison of single-cell transcriptional variation within and between ILC populations provides new insight into ILC biology during homeostasis, with additional implications for dysregulation of the immune system.
Place, publisher, year, edition, pages
2016. Vol. 17, no 4, 451-460 p.
Immunology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-286647DOI: 10.1038/ni.3368ISI: 000372508000019PubMedID: 26878113OAI: oai:DiVA.org:uu-286647DiVA: diva2:923822
FunderSwedish Research CouncilSwedish Cancer SocietySwedish Society for Medical Research (SSMF)Swedish Foundation for Strategic Research Torsten Söderbergs stiftelseStockholm County CouncilEU, FP7, Seventh Framework Programme, 655677