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The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Ludwig Inst Canc Res, S-10401 Stockholm, Sweden.;Karolinska Inst, Dept Cell & Mol Biol, Stockholm, Sweden..
Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden..
Ludwig Inst Canc Res, S-10401 Stockholm, Sweden..
Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden..
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2016 (English)In: Nature Immunology, ISSN 1529-2908, E-ISSN 1529-2916, Vol. 17, no 4, 451-460 p.Article in journal (Refereed) PublishedText
Abstract [en]

Innate lymphoid cells (ILCs) are increasingly appreciated as important participants in homeostasis and inflammation. Substantial plasticity and heterogeneity among ILC populations have been reported. Here we have delineated the heterogeneity of human ILCs through single-cell RNA sequencing of several hundreds of individual tonsil CD127(+) ILCs and natural killer (NK) cells. Unbiased transcriptional clustering revealed four distinct populations, corresponding to ILC1 cells, ILC2 cells, ILC3 cells and NK cells, with their respective transcriptomes recapitulating known as well as unknown transcriptional profiles. The single-cell resolution additionally divulged three transcriptionally and functionally diverse subpopulations of ILC3 cells. Our systematic comparison of single-cell transcriptional variation within and between ILC populations provides new insight into ILC biology during homeostasis, with additional implications for dysregulation of the immune system.

Place, publisher, year, edition, pages
2016. Vol. 17, no 4, 451-460 p.
National Category
Immunology in the medical area
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URN: urn:nbn:se:uu:diva-286647DOI: 10.1038/ni.3368ISI: 000372508000019PubMedID: 26878113OAI: oai:DiVA.org:uu-286647DiVA: diva2:923822
Funder
Swedish Research CouncilSwedish Cancer SocietySwedish Society for Medical Research (SSMF)Swedish Foundation for Strategic Research Torsten Söderbergs stiftelseStockholm County CouncilEU, FP7, Seventh Framework Programme, 655677
Available from: 2016-04-27 Created: 2016-04-21 Last updated: 2016-04-27Bibliographically approved

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Björklund, Åsa K.
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