Gene expression of cytochrome P450 1B1 and 2D6 in leukocytes in human pregnancy
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Women's and Children's HealthDepartment of Medical Sciences2003 (English)In: Pharmacology and Toxicology, ISSN 0901-9928, E-ISSN 1600-0773, Vol. 92, no 6, 295-9 p.Article in journal (Refereed) Published
We investigated the influence of human pregnancy on gene expression of two cytochrome P450 enzymes in white blood cells. Cytochrome P450 1B1 (CYP1B1) catalyses oestradiol 4-hydroxylation, and may participate in the endocrine regulation of oestrogens. Cytochrome P450 2D6 (CYP2D6) metabolises many commonly used drugs, and previous studies have suggested that it is induced during pregnancy. CYP1B1 and CYP2D6 were therefore considered to be of interest in human pregnancy. As it is not ethically possible to take liver biopsies from healthy mothers during pregnancy, easily accessible cells that express the genes were used as a surrogate tissue. White blood cells were collected from eighteen pregnant women, and were used to measure CYP1B1 and CYP2D6 ribonucleic acid (RNA). The analysis was repeated after pregnancy, the women, thus, serving as their own controls. Real-time reverse transcriptase - polymerase chain reaction methods were used with 18S ribosomal RNA as an internal control. A slight, but not significant, increase in gene activity of CYP1B1 was detected during pregnancy. Expression of CYP2D6 in blood was extremely low, and induction of CYP2D6 during pregnancy could not be confirmed. In conclusion, gene expression of CYP1B1 and CYP2D6 in leukocytes was not significantly up-regulated in the third trimester of pregnancy, but a trend indicating an altered metabolism during pregnancy was detected.
Place, publisher, year, edition, pages
2003. Vol. 92, no 6, 295-9 p.
pregnancy, CYP2D6, CYP1B1
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-64512DOI: 10.1034/j.1600-0773.2003.920607.xPubMedID: 12787262 OAI: oai:DiVA.org:uu-64512DiVA: diva2:92423