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Applications of nanospray desorption electrospray ionization mass spectrome: Analysis of lipids and metabolites in brain tissue sections and single cells
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
2016 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Uppsala: Department of Chemistry, Uppsala University , 2016. , 37 p.
Keyword [en]
Nano-DESI, single cell analysis, small molecule neurotransmitters, mass spectrometry imaging, MSI, GABA, Glutamate, Acetylcholine
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-291339OAI: oai:DiVA.org:uu-291339DiVA: diva2:925383
Presentation
2016-05-31, B7:113a, Husargatan 3, Uppsala, 10:15 (English)
Opponent
Supervisors
Available from: 2016-11-28 Created: 2016-05-01 Last updated: 2016-11-28Bibliographically approved
List of papers
1. Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
Open this publication in new window or tab >>Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
2016 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 141, no 12, 3686-3695 p.Article in journal (Refereed) Published
Abstract [en]

Small molecule neurotransmitters are essential for the function of the nervous system, and neurotransmitter imbalances are often connected to neurological disorders. The ability to quantify such imbalances is important to provide insights into the biochemical mechanisms underlying the disorder. This proof-of-principle study presents online quantification of small molecule neurotransmitters, specifically acetylcholine, γ-aminobutyric acid (GABA) and glutamate, in rat brain tissue sections using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging. By incorporating deuterated internal standards in the nano-DESI solvent we show identification, accurate mapping, and quantification of these small neurotransmitters in rat brain tissue without introducing any additional sample preparation steps. We find that GABA is about twice as abundant in the medial septum-diagonal band complex (MSDB) as in the cortex, while glutamate is about twice as abundant in the cortex as compared to the MSDB. The study shows that nano-DESI is well suited for imaging of small molecule neurotransmitters in health and disease.

National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-281314 (URN)10.1039/c5an02620b (DOI)000378942900021 ()26859000 (PubMedID)
Funder
Swedish Research Council, VR 621-2013-4231Swedish Foundation for Strategic Research , SSF ICA-6
Available from: 2016-03-22 Created: 2016-03-22 Last updated: 2016-11-28Bibliographically approved
2. Detection of endogenous lipids and metabolites in single cells using nano-DESI
Open this publication in new window or tab >>Detection of endogenous lipids and metabolites in single cells using nano-DESI
(English)Manuscript (preprint) (Other academic)
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-291338 (URN)
Available from: 2016-05-01 Created: 2016-05-01 Last updated: 2016-11-28

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